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Journal of Virology, September 2007, p. 9556-9559, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.01042-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation{triangledown}

Makoto Ozawa,1,2 Hideo Goto,1,2 Taisuke Horimoto,1,2 and Yoshihiro Kawaoka1,2,3*

Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan,1 Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan,2 Department of Pathological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin 537063

Received 14 May 2007/ Accepted 18 June 2007

Plasmid-based reverse genetics systems allow the generation of influenza A virus entirely from cloned cDNA. However, since the efficiency of virus generation is dependent on the plasmid transfection efficiency of cells, virus generation is difficult in cells approved for vaccine production that have low transfection efficiencies (e.g., Vero cells). Here we established an alternative reverse genetics system for influenza virus generation by using an adenovirus vector (AdV) which achieves highly efficient gene transfer independent of cell transfection efficiency. This AdV-mediated reverse genetics system will be useful for generating vaccine seed strains and for basic influenza virus studies.

* Corresponding author. Mailing address: Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-03-5449-5310. Fax: 81-03-5449-5408. E-mail: kawaoka{at}ims.u-tokyo.ac.jp

{triangledown} Published ahead of print on 27 June 2007.

Journal of Virology, September 2007, p. 9556-9559, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.01042-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Murakami, S., Horimoto, T., Yamada, S., Kakugawa, S., Goto, H., Kawaoka, Y. (2008). Establishment of Canine RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus: Its Application for H5N1 Vaccine Production. J. Virol. 82: 1605-1609 [Abstract] [Full Text]  


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