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Journal of Virology, September 2007, p. 9461-9468, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00735-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Gag Influences Transformation by Abelson Murine Leukemia Virus and Suppresses Nuclear Localization of the v-Abl Protein{triangledown}

Chae-Ryun Yi1,2 and Naomi Rosenberg1,2,3*

Molecular Microbiology Graduate Program, Sackler School of Graduate Biomedical Sciences,1 Departments of Molecular Biology and Microbiology,2 Pathology, Tufts Medical School, Boston, Massachusetts 021113

Received 4 April 2007/ Accepted 18 June 2007

Like the v-Onc proteins encoded by many transforming retroviruses, the v-Abl protein is expressed as a Gag-Onc fusion. Although the Gag-derived myristoylation signal targets the v-Abl protein to the plasma membrane, the protein contains the entire MA and p12 sequences and a small number of CA-derived residues. To understand the role of Gag sequences in transformation, mutants lacking portions of these sequences were examined for the effects of these deletions on v-Abl function and localization. Deletion of the N-terminal third of p12 or all of p12 enhanced the transformation of both pre-B cells and NIH 3T3 cells. In contrast, deletions in MA or a deletion removing all of Gag except the first 34 amino acids important for myristoylation highly compromised the ability to transform either cell type. Although all of the mutant proteins retained kinase activity, those defective in transformation were reduced in their ability to activate Erk, suggesting a role for Gag sequences in v-Abl signaling. Immunofluorescence analysis revealed that a v-Abl protein retaining only the first 34 amino acids of Gag localized to the nucleus. These data indicate that Gag sequences are important for normal v-Abl signaling and that they suppress nuclear localization of the molecule.

* Corresponding author. Mailing address: Sackler 814, Tufts Medical School, 136 Harrison Avenue, Boston, MA 02111. Phone: (617) 636-2143. Fax: (617) 636-0337. E-mail: naomi.rosenberg{at}tufts.edu

{triangledown} Published ahead of print on 27 June 2007.

Journal of Virology, September 2007, p. 9461-9468, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00735-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yi, C.-r., Rosenberg, N. (2008). Mutations Affecting the MA Portion of the v-Abl Protein Reveal a Conserved Role of Gag in Abelson Murine Leukemia Virus (MLV) and Moloney MLV. J. Virol. 82: 5307-5315 [Abstract] [Full Text]  


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