This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deng, F.
Right arrow Articles by Hu, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deng, F.
Right arrow Articles by Hu, Z.

 Previous Article  |  Next Article 

Journal of Virology, September 2007, p. 9377-9385, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00632-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Proteomics Analysis of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Identified Two New Occlusion-Derived Virus-Associated Proteins, HA44 and HA100{triangledown}

Fei Deng,1,{dagger} Ranran Wang,1,{dagger} Minggang Fang,1,{ddagger} Yue Jiang,1 Xushi Xu,1 Hanzhong Wang,1 Xinwen Chen,1 Basil M. Arif,2 Lin Guo,3 Hualin Wang,1 and Zhihong Hu1*

State Key Laboratory of Virology and Joint Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China,1 Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste. Marie, Ontario, Canada,2 State Key Laboratory of Virology, Wuhan University and College of Life Sciences, Wuhan 430072, People's Republic of China3

Received 24 March 2007/ Accepted 14 June 2007

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry were used to analyze the structural proteins of the occlusion-derived virus (ODV) of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV), a group II NPV. Twenty-three structural proteins of HearNPV ODV were identified, 21 of which have been reported previously as structural proteins or ODV-associated proteins in other baculoviruses. These include polyhedrin, P78/83, P49, ODV-E18, ODV-EC27, ODV-E56, P74, LEF-3, HA66 (AC66), DNA polymerase, GP41, VP39, P33, ODV-E25, helicase, P6.9, ODV/BV-C42, VP80, ODV-EC43, ODV-E66, and PIF-1. Two proteins encoded by HearNPV ORF44 (ha44) and ORF100 (ha100) were discovered as ODV-associated proteins for the first time. ha44 encodes a protein of 378 aa with a predicted mass of 42.8 kDa. ha100 encodes a protein of 510 aa with a predicted mass of 58.1 kDa and is a homologue of the gene for poly(ADP-ribose) glycohydrolase (parg). Western blot analysis and immunoelectron microscopy confirmed that HA44 is associated with the nucleocapsid and HA100 is associated with both the nucleocapsid and the envelope of HearNPV ODV. HA44 is conserved in group II NPVs and granuloviruses but does not exist in group I NPVs, while HA100 is conserved only in group II NPVs.

* Corresponding author. Mailing address: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China. Phone and fax: 86-27-87197180. E-mail: huzh{at}wh.iov.cn

{triangledown} Published ahead of print on 20 June 2007.

{dagger} F.D. and R.W. contributed equally to this work.

{ddagger} Present address: Department of Agroecology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.

Journal of Virology, September 2007, p. 9377-9385, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00632-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • de Jong, J., Arif, B. M., Theilmann, D. A., Krell, P. J. (2009). Autographa californica Multiple Nucleopolyhedrovirus me53 (ac140) Is a Nonessential Gene Required for Efficient Budded-Virus Production. J. Virol. 83: 7440-7448 [Abstract] [Full Text]  
  • Tan, Y., Bideshi, D. K., Johnson, J. J., Bigot, Y., Federici, B. A. (2009). Proteomic analysis of the Spodoptera frugiperda ascovirus 1a virion reveals 21 proteins. J. Gen. Virol. 90: 359-365 [Abstract] [Full Text]  
  • Wu, W., Liang, H., Kan, J., Liu, C., Yuan, M., Liang, C., Yang, K., Pang, Y. (2008). Autographa californica Multiple Nucleopolyhedrovirus 38K Is a Novel Nucleocapsid Protein That Interacts with VP1054, VP39, VP80, and Itself. J. Virol. 82: 12356-12364 [Abstract] [Full Text]  
  • Tan, Y., Jiang, L., Wang, M., Yin, F., Deng, F., Liu, M., Hu, Z., Wang, H. (2008). Mutagenesis and Nuclear Magnetic Resonance Analyses of the Fusion Peptide of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus F Protein. J. Virol. 82: 8138-8148 [Abstract] [Full Text]  
  • Simon, O., Williams, T., Asensio, A. C., Ros, S., Gaya, A., Caballero, P., Possee, R. D. (2008). Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies. J. Virol. 82: 7897-7904 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»