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Journal of Virology, September 2007, p. 9072-9077, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00587-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Environment Determines Fidelity for an RNA Virus Replicase{triangledown}

Justin S. Pita, Joachim R. de Miranda,{dagger} William L. Schneider,{ddagger} and Marilyn J. Roossinck*

Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73402

Received 20 March 2007/ Accepted 25 May 2007

The rate of insertion and deletion mutations of the replicase of Cucumber mosaic virus (CMV) was determined in planta by using a parasitic satellite RNA (satRNA) as a reporter. We found that the CMV replicase had different fidelity in different environments, with important implications in viral disease evolution. Insertions were very rare events, irrespective of the region of the satRNA genome assayed and independent of the hosts tested. On the other hand, deletion events were more frequent but were restricted to a highly structured region of the reporter. Deletion mutation rates were different for the two hosts tested, although the mutation distribution was not influenced by the hosts. Moreover, hot spots with high mutation rates were identified on the satRNA genome.

* Corresponding author. Mailing address: Plant Biology Division, The Samuel Roberts Noble Foundation, P.O. Box 2180, Ardmore, Oklahoma 73402. Phone: (580) 224-6630. Fax: (580) 224-6692. E-mail: mroossinck{at}noble.org

{triangledown} Published ahead of print on 6 June 2007.

{dagger} Present address: School of Biological Sciences, Queen's University Belfast, BT9 7BL Belfast, Northern Ireland.

{ddagger} Present address: USDA-ARS, 1301 Ditto Ave., Frederick, MD 21702.

Journal of Virology, September 2007, p. 9072-9077, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00587-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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