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Journal of Virology, September 2007, p. 8944-8952, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00180-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Natural Recombination Event within the Capsid Genomic Region Leading to a Chimeric Strain of Human Enterovirus B{triangledown}

Lamjed Bouslama,1,{dagger} Dorsaf Nasri,1,2,{dagger} Lionel Chollet,3 Khaoula Belguith,1 Thomas Bourlet,2 Mahjoub Aouni,1 Bruno Pozzetto,2* and Sylvie Pillet2

Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, Monastir, Tunisia,1 Laboratory of Bacteriology-Virology, GIMAP EA3064, Faculty of Medicine of Saint-Etienne, Saint-Etienne, France,2 Laboratory of Bacteriology-Virology, Hospital of Toulon-La Seyne sur Mer, Toulon-La Seyne sur Mer, France3

Received 26 January 2007/ Accepted 21 May 2007

Recombination between two strains is a known phenomenon for enteroviruses replicating within a single cell. We describe a recombinant strain recovered from human stools, typed as coxsackievirus B4 (CV-B4) and CV-B3 after partial sequencing of the VP1 and VP2 coding regions, respectively. The strain was neutralized by a polyclonal CV-B3-specific antiserum but not by a CV-B4-specific antiserum. The nucleotide sequence analysis of the whole structural genomic region showed the occurrence of a recombination event at position 1950 within the VP3 capsid gene, in a region coding for the 2b antigenic site previously described for CV-B3. This observation evidences for the first time the occurrence of an interserotypic recombination within the VP2-VP3-VP1 capsid region between two nonpoliovirus enterovirus strains. The neutralization pattern suggests that the major antigenic site is located within the VP2 protein.

* Corresponding author. Mailing address: Laboratory of Bacteriology-Virology, GIMAP EA3064, 15 rue Ambroise Paré, Faculté de Médecine Jacques Lisfranc, 42023 Saint-Etienne Cedex 02, France. Phone: 33 4 77 82 84 34. Fax: 33 4 77 82 84 60. E-mail: Bruno.Pozzetto{at}univ-st-etienne.fr

{triangledown} Published ahead of print on 30 May 2007.

{dagger} Lamjed Bouslama and Dorsaf Nasri contributed equally to this work.

Journal of Virology, September 2007, p. 8944-8952, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00180-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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