This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, J.-H.
Right arrow Articles by Wu, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, J.-H.
Right arrow Articles by Wu, L.

 Previous Article  |  Next Article 

Journal of Virology, September 2007, p. 8933-8943, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00878-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Functionally Distinct Transmission of Human Immunodeficiency Virus Type 1 Mediated by Immature and Mature Dendritic Cells{triangledown}

Jian-Hua Wang, Alicia M. Janas, Wendy J. Olson,{dagger} and Li Wu*

Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226

Received 24 April 2007/ Accepted 3 June 2007

Dendritic cells (DCs) potently stimulate the transmission of human immunodeficiency virus type 1 (HIV-1) to CD4+ T cells. Immature DCs (iDCs) located in submucosal tissues can capture HIV-1 and migrate to lymphoid tissues, where they become mature DCs (mDCs) for effective antigen presentation. DC maturation promotes HIV-1 transmission; however, the underlying mechanisms remain unclear. Here we have compared monocyte-derived iDCs and mDCs for their efficiencies and mechanisms of HIV-1 transmission. We have found that mDCs significantly facilitate HIV-1 endocytosis and efficiently concentrate HIV-1 at virological synapses, which contributes to mDC-enhanced viral transmission, at least in part. mDCs were more efficient than iDCs in transferring HIV-1 to various types of target cells independently of C-type lectins, which partially accounted for iDC-mediated HIV-1 transmission. Efficient HIV-1 trans-infection mediated by iDCs and mDCs required contact between DCs and target cells. Moreover, rapid HIV-1 degradation occurred in both iDCs and mDCs, which correlated with the lack of HIV-1 retention-mediated long-term viral transmission. Our results provide new insights into the mechanisms underlying DC-mediated HIV-1 transmission, suggesting that HIV-1 exploits mDCs to facilitate its dissemination within lymphoid tissues.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226. Phone: (414) 456-4075. Fax: (414) 456-6535. E-mail: liwu{at}mcw.edu

{triangledown} Published ahead of print on 13 June 2007.

{dagger} Present address: University of Wisconsin—Milwaukee, Milwaukee, WI 53201.

Journal of Virology, September 2007, p. 8933-8943, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00878-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Anand, A. R., Prasad, A., Bradley, R. R., Deol, Y. S., Nagaraja, T., Ren, X., Terwilliger, E. F., Ganju, R. K. (2009). HIV-1 gp120-induced migration of dendritic cells is regulated by a novel kinase cascade involving Pyk2, p38 MAP kinase, and LSP1. Blood 114: 3588-3600 [Abstract] [Full Text]  
  • Wang, J.-H., Kwas, C., Wu, L. (2009). Intercellular Adhesion Molecule 1 (ICAM-1), but Not ICAM-2 and -3, Is Important for Dendritic Cell-Mediated Human Immunodeficiency Virus Type 1 Transmission. J. Virol. 83: 4195-4204 [Abstract] [Full Text]  
  • Hatch, S. C., Archer, J., Gummuluru, S. (2009). Glycosphingolipid Composition of Human Immunodeficiency Virus Type 1 (HIV-1) Particles Is a Crucial Determinant for Dendritic Cell-Mediated HIV-1 trans-Infection. J. Virol. 83: 3496-3506 [Abstract] [Full Text]  
  • Dong, C., Kwas, C., Wu, L. (2009). Transcriptional Restriction of Human Immunodeficiency Virus Type 1 Gene Expression in Undifferentiated Primary Monocytes. J. Virol. 83: 3518-3527 [Abstract] [Full Text]  
  • Coutant, J., Yu, H., Clement, M.-J., Alfsen, A., Toma, F., Curmi, P. A., Bomsel, M. (2008). Both lipid environment and pH are critical for determining physiological solution structure of 3-D-conserved epitopes of the HIV-1 gp41-MPER peptide P1. FASEB J. 22: 4338-4351 [Abstract] [Full Text]  
  • Nightingale, Z. D., Patkar, C., Rothman, A. L. (2008). Viral replication and paracrine effects result in distinct, functional responses of dendritic cells following infection with dengue 2 virus. J. Leukoc. Biol. 84: 1028-1038 [Abstract] [Full Text]  
  • Frank, I., Stossel, H., Gettie, A., Turville, S. G., Bess, J. W. Jr., Lifson, J. D., Sivin, I., Romani, N., Robbiani, M. (2008). A Fusion Inhibitor Prevents Spread of Immunodeficiency Viruses, but Not Activation of Virus-Specific T Cells, by Dendritic Cells. J. Virol. 82: 5329-5339 [Abstract] [Full Text]  
  • Forthal, D. N., Landucci, G., Bream, J., Jacobson, L. P., Phan, T. B., Montoya, B. (2007). Fc{gamma}RIIa Genotype Predicts Progression of HIV Infection. J. Immunol. 179: 7916-7923 [Abstract] [Full Text]  
  • Dong, C., Janas, A. M., Wang, J.-H., Olson, W. J., Wu, L. (2007). Characterization of Human Immunodeficiency Virus Type 1 Replication in Immature and Mature Dendritic Cells Reveals Dissociable cis- and trans-Infection. J. Virol. 81: 11352-11362 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»