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Journal of Virology, August 2007, p. 8814-8820, Vol. 81, No. 16
0022-538X/07/$08.00+0     doi:10.1128/JVI.02824-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Monitoring the Antiviral Effect of Alpha Interferon on Individual Cells{triangledown}

Chon Saeng Kim,1 Jong Ha Jung,1 Takaji Wakita,2 Seung Kew Yoon,3 and Sung Key Jang1*

PBC, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea,1 Department of Virology II, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan,2 Department of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea3

Received 21 December 2006/ Accepted 21 May 2007

An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-{alpha}) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-{alpha} were observed among HCV-containing cells.

* Corresponding author. Mailing address: PBC, Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang 790-784, Republic of Korea. Phone: 82-54-279-2298. Fax: 82-54-279-8009. E-mail: sungkey{at}postech.ac.kr

{triangledown} Published ahead of print on 30 May 2007.

Journal of Virology, August 2007, p. 8814-8820, Vol. 81, No. 16
0022-538X/07/$08.00+0     doi:10.1128/JVI.02824-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pawlotsky, J.-M. (2009). Review: Therapeutic implications of hepatitis C virus resistance to antiviral drugs. Therapeutic Advances in Gastroenterology 2: 205-219 [Abstract]  
  • Paek, K. Y., Kim, C. S., Park, S. M., Kim, J. H., Jang, S. K. (2008). RNA-Binding Protein hnRNP D Modulates Internal Ribosome Entry Site-Dependent Translation of Hepatitis C Virus RNA. J. Virol. 82: 12082-12093 [Abstract] [Full Text]  
  • Randall, R. E., Goodbourn, S. (2008). Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J. Gen. Virol. 89: 1-47 [Abstract] [Full Text]  


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