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Journal of Virology, August 2007, p. 8579-8586, Vol. 81, No. 16
0022-538X/07/$08.00+0     doi:10.1128/JVI.00263-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Protein Kinase A-Dependent Mechanism by Which Rotavirus Affects the Distribution and mRNA Level of the Functional Tight Junction-Associated Protein, Occludin, in Human Differentiated Intestinal Caco-2 Cells{triangledown}

Isabelle Beau,1,2,{dagger} Jacqueline Cotte-Laffitte,1,2,{dagger} Raymonde Amsellem,1,2 and Alain L. Servin1,2*

INSERM, UMR 756, Châtenay-Malabry, France,1 Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry, France2

Received 7 February 2007/ Accepted 24 May 2007

We found that at the tight junctions (TJs) of Caco-2 cell monolayers, rhesus monkey rotavirus (RRV) infection induced the disappearance of occludin. Confocal laser scanning microscopy showed the disappearance of occludin from the cell-cell boundaries without modifying the expression of the other TJ-associated proteins, ZO-1 and ZO-3. Western immunoblot analysis of RRV-infected cells showed a significant fall in the levels of the nonphosphorylated form of occludin in both Triton X-100-insoluble and Triton X-100-soluble fractions, without any change in the levels of the phosphorylated form of occludin. Quantitative reverse transcription-PCRs revealed that the level of transcription of the gene that encodes occludin was significantly reduced in RRV-infected cells. Treatment of RRV-infected cells with Rp-cyclic AMP and protein kinase A inhibitors H89 and KT5720 during the time course of the infection restored the distribution of occludin and a normal level of transcription of the gene that encodes occludin.

* Corresponding author. Mailing address: Faculté de Pharmacie, INSERM Unit 510, Châtenay-Malabry, France 92296. Phone: 33 1 46 83 56 61. Fax: 33 1 46 83 58 44. E-mail: alain.servin{at}u-psud.fr

{triangledown} Published ahead of print on 6 June 2007.

{dagger} I. Beau and J. Cotte-Laffitte contributed equally to this work.

Journal of Virology, August 2007, p. 8579-8586, Vol. 81, No. 16
0022-538X/07/$08.00+0     doi:10.1128/JVI.00263-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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