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Journal of Virology, August 2007, p. 8337-8340, Vol. 81, No. 15
0022-538X/07/$08.00+0     doi:10.1128/JVI.00520-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Herpes Simplex Virus gD-YFP Fusion Glycoprotein Is Transported Separately from Viral Capsids in Neuronal Axons{triangledown}

Aleksandra Snyder,1* Birgitte Bruun,2 Helena M. Browne,2 and David C. Johnson1

Department of Molecular Microbiology and Immunology, Oregon Health & Sciences University, Portland, Oregon 97239,1 Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom2

Received 12 March 2007/ Accepted 15 May 2007

Two models describing how alphaherpesviruses exit neurons differ with respect to whether nucleocapsids and envelope glycoproteins travel toward axon termini separately or as assembled enveloped virions. Recently, a pseudorabies virus glycoprotein D (gD)-green fluorescent protein fusion was found to colocalize with viral capsids, supporting anterograde transport of enveloped virions. Previous antibody staining experiments demonstrated that herpes simplex virus (HSV) glycoproteins and capsids are separately transported in axons. Here, we generated an HSV expressing a gD-yellow fluorescent protein (YFP) fusion and found that gD-YFP and capsids were transported separately in neuronal axons. Anti-gD antibodies colocalized with gD-YFP, indicating that gD-YFP behaves like wild-type HSV gD.

* Corresponding author. Mailing address: L-220, Department of Microbiology & Immunology, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd., Portland, OR 97239. Phone: (503) 494-0834. Fax: (503) 494-6862. E-mail: snyderal{at}ohsu.edu

{triangledown} Published ahead of print on 23 May 2007.

Journal of Virology, August 2007, p. 8337-8340, Vol. 81, No. 15
0022-538X/07/$08.00+0     doi:10.1128/JVI.00520-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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