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Journal of Virology, August 2007, p. 7852-7859, Vol. 81, No. 15
0022-538X/07/$08.00+0     doi:10.1128/JVI.02203-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Selection of Mutations in the Connection and RNase H Domains of Human Immunodeficiency Virus Type 1 Reverse Transcriptase That Increase Resistance to 3'-Azido-3'-Dideoxythymidine{triangledown}

Jessica H. Brehm,1 Dianna Koontz,1 Jeffrey D. Meteer,1 Vinay Pathak,2 Nicolas Sluis-Cremer,1 and John W. Mellors1*

Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261,1 National Cancer Institute, Frederick, Maryland 217022

Received 6 October 2006/ Accepted 5 May 2007

Recent work indicates that mutations in the C-terminal domains of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) increase 3'-azido-3'-dideoxythymidine (AZT) resistance. Because it is not known whether AZT selects for mutations outside of the polymerase domain of RT, we carried out in vitro experiments in which HIV-1LAI or AZT-resistant HIV-1LAI (M41L/L210W/T215Y) was passaged in MT-2 cells in increasing concentrations of AZT. The first resistance mutations to appear in HIV-1LAI were two polymerase domain thymidine analog mutations (TAMs), D67N and K70R, and two novel mutations, A371V in the connection domain and Q509L in the RNase H domain, that together conferred up to 90-fold AZT resistance. Thereafter, the T215I mutation appeared but was later replaced by T215F, resulting in a large increase in AZT resistance (~16,000-fold). Mutations in the connection and RNase H domains were not selected starting with AZT-resistant virus (M41L/L210W/T215Y). The roles of A371V and Q509L in AZT resistance were confirmed by site-directed mutagenesis: A371V and Q509L together increased AZT resistance ~10- to 50-fold in combination with TAMs (M41L/L210W/T215Y or D67N/K70R/T215F) but had a minimal effect without TAMs (1.7-fold). A371V and Q509L also increased cross-resistance with TAMs to lamivudine and abacavir, but not stavudine or didanosine. These results provide the first evidence that mutations in the connection and RNase H domains of RT can be selected in vitro by AZT and confer greater AZT resistance and cross-resistance to nucleoside RT inhibitors in combination with TAMs in the polymerase domain.

* Corresponding author. Mailing address: University of Pittsburgh School of Medicine, S818 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261. Phone: (412) 624-8512. Fax: (412) 383-7982. E-mail: mellors{at}dom.pitt.edu

{triangledown} Published ahead of print on 16 May 2007.

Journal of Virology, August 2007, p. 7852-7859, Vol. 81, No. 15
0022-538X/07/$08.00+0     doi:10.1128/JVI.02203-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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