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Journal of Virology, July 2007, p. 7811-7815, Vol. 81, No. 14
0022-538X/07/$08.00+0     doi:10.1128/JVI.00050-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Entry of Rice Dwarf Virus into Cultured Cells of Its Insect Vector Involves Clathrin-Mediated Endocytosis{triangledown}

Taiyun Wei,1 Hongyan Chen,1,2 Tamaki Ichiki-Uehara,1 Hiroyuki Hibino,1 and Toshihiro Omura1*

Laboratory of Virology, National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan,1 Biotechnology Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, People's Republic of China2

Received 8 January 2007/ Accepted 20 April 2007

Electron microscopy revealed that the entry of Rice dwarf virus (RDV) into insect vector cells involved endocytosis via coated pits. The treatment of cells with drugs that block receptor-mediated or clathrin-mediated endocytosis significantly reduced RDV infectivity. However, the drug that blocks caveola-mediated endocytosis had a negligible effect on such infection. Infection was also inhibited when cells had been pretreated with bafilomycin A1, which interferes with acidification of endosomes. Moreover, immunofluorescence staining indicated that the virus is internalized into early endosomes. Together, our data indicate that RDV enters insect vector cells through receptor-mediated, clathrin-dependent endocytosis and is sequestered in early endosomes.

* Corresponding author. Mailing address: National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan. Phone: 81-29-838-8932. Fax: 81-29-838-8929. E-mail: toomura{at}affrc.go.jp

{triangledown} Published ahead of print on 2 May 2007.

Journal of Virology, July 2007, p. 7811-7815, Vol. 81, No. 14
0022-538X/07/$08.00+0     doi:10.1128/JVI.00050-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wei, T., Hibino, H., Omura, T. (2008). Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells. J. Gen. Virol. 89: 2915-2920 [Abstract] [Full Text]  
  • Acosta, E. G., Castilla, V., Damonte, E. B. (2008). Functional entry of dengue virus into Aedes albopictus mosquito cells is dependent on clathrin-mediated endocytosis. J. Gen. Virol. 89: 474-484 [Abstract] [Full Text]  
  • Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y. (2007). The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc. Natl. Acad. Sci. USA 104: 19547-19552 [Abstract] [Full Text]  


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