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Journal of Virology, July 2007, p. 7695-7701, Vol. 81, No. 14
0022-538X/07/$08.00+0     doi:10.1128/JVI.00282-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Stable 2-Kilobase Latency-Associated Transcript of Herpes Simplex Virus Type 1 Can Alter the Assembly of the 60S Ribosomal Subunit and Is Exported from Nucleus to Cytoplasm by a CRM1-Dependent Pathway{triangledown}

Doina Atanasiu and Nigel W. Fraser*

Department of Microbiology, University of Pennsylvania School of Medicine, 3610 Hamilton Walk, Philadelphia, Pennsylvania 19104

Received 9 February 2007/ Accepted 30 April 2007

During latency of herpes simplex virus type 1 in the neurons of the peripheral nervous system, the major transcript detected is the 2-kb latency-associated transcript (LAT) intron. During lytic infection, this intron has been shown to associate with ribosomes, suggesting a role in modifying the translational machinery of infected cells. In this study we show, using LAT-transfected cells, that the interaction of the intron with the 60S ribosomal subunit leads to irreversible changes in the sedimentation profile of this subunit in the nucleus. Furthermore, the 2-kb LAT intron is transported to the cytoplasm as part of the 60S ribosomal subunit, using a CRM1-dependent pathway.

* Corresponding author. Mailing address: Department of Microbiology, University of Pennsylvania School of Medicine, 3610 Hamilton Walk, Philadelphia, PA 19104. Phone: (215) 898-3847. Fax: (215) 898-3849. E-mail: nfraser{at}mail.med.upenn.edu

{triangledown} Published ahead of print on 9 May 2007.

Journal of Virology, July 2007, p. 7695-7701, Vol. 81, No. 14
0022-538X/07/$08.00+0     doi:10.1128/JVI.00282-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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