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Journal of Virology, July 2007, p. 7380-7387, Vol. 81, No. 14
0022-538X/07/$08.00+0     doi:10.1128/JVI.00222-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Type 1 Cytoplasmic Envelopment Requires Functional Vps4

Colin M. Crump,^* Catherine Yates, and Tony Minson

Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom

Received 1 February 2007/ Accepted 3 May 2007

The assembly and egress of herpesviruses are complex processes that require the budding of viral nucleocapsids into the lumen of cytoplasmic compartments to form mature infectious virus. This envelopment stage shares many characteristics with the formation of luminal vesicles in multivesicular endosomes. Through expression of dominant-negative Vps4, an enzyme that is essential for the formation of luminal vesicles in multivesicular endosomes, we now show that Vps4 function is required for the cytoplasmic envelopment of herpes simplex virus type 1. This is the first example of a large enveloped DNA virus engaging the multivesicular endosome sorting machinery to enable infectious virus production.

Published ahead of print on 16 May 2007.

Catherine Yates sadly died during the preparation of the manuscript.

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