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Journal of Virology, July 2007, p. 7316-7319, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00545-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cytokine-Independent Upregulation of MDA5 in Viral Infection{triangledown}

Jacob S. Yount, Thomas M. Moran, and Carolina B. López*

Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029

Received 14 March 2007/ Accepted 23 April 2007

The RNA helicases RIG-I and MDA5 detect virus infection of dendritic cells (DCs) leading to cytokine induction. Maximal sensitivity for virus detection by these helicases is obtained after their upregulation, which is thought to occur primarily through type I interferon (IFN) signaling. Here we demonstrate that in response to paramyxovirus infection, RIG-I upregulation requires type I IFN whereas MDA5 expression is increased by Sendai virus infection independently of signaling mediated by type I IFN, STAT1, tumor necrosis factor alpha, or NF-{kappa}B. This MDA5 upregulation is largely lost in IRF3 knockout DCs and is achieved in type I IFN-deficient cells expressing constitutively active IRF3.

* Corresponding author. Mailing address: Department of Microbiology, The Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1124, New York, NY 10029. Phone: (212) 241-3949. Fax: (212) 987-3653. E-mail: Carolina.Lopez{at}mssm.edu

{triangledown} Published ahead of print on 2 May 2007.

Journal of Virology, July 2007, p. 7316-7319, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00545-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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