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Journal of Virology, July 2007, p. 7297-7299, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02468-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The X Protein of Borna Disease Virus Serves Essential Functions in the Viral Multiplication Cycle

Marion Poenisch, Sandra Wille, Andreas Ackermann, Peter Staeheli,^* and Urs Schneider^*

Department of Virology, University of Freiburg, 79104 Freiburg, Germany

Received 9 November 2006/ Accepted 3 April 2007

The X gene of Borna disease virus (BDV) encodes a nonstructural 10-kDa protein that can interact with viral polymerase cofactor P, thus regulating polymerase activity. It remained unknown whether X is essential for virus multiplication. All our attempts to generate mutant BDV with a nonfunctional X gene proved unsuccessful. However, a mutant virus with an inactive X gene was able to replicate in Vero cells if an artificial gene cassette encoding X was inserted at a site near the 5' end of the viral genome. These results indicate that X performs essential viral functions.

Published ahead of print on 11 April 2007.

These two authors contributed equally to this work.

This article has been cited by other articles:

• Poenisch, M., Wille, S., Staeheli, P., Schneider, U. (2008). Polymerase Read-Through at the First Transcription Termination Site Contributes to Regulation of Borna Disease Virus Gene Expression. J. Virol. 82: 9537-9545 [Abstract] [Full Text]  
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