JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JVI.00406-07v1
81/13/7280    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, H.
Right arrow Articles by Shioda, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, H.
Right arrow Articles by Shioda, T.

 Previous Article  |  Next Article 

Journal of Virology, July 2007, p. 7280-7285, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00406-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Single Amino Acid of the Human Immunodeficiency Virus Type 2 Capsid Affects Its Replication in the Presence of Cynomolgus Monkey and Human TRIM5{alpha}s{triangledown}

Haihan Song,1 Emi E. Nakayama,1 Masaru Yokoyama,2 Hironori Sato,2 Jay A. Levy,3 and Tatsuo Shioda1*

Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan,1 Laboratory of Viral Genomics, Center for Pathogen Genomics, National Institute of Infectious Diseases, Tokyo 208-0011, Japan,2 Cancer Research Institute, School of Medicine, University of California, San Francisco, California 941433

Received 26 February 2007/ Accepted 23 April 2007

Human immunodeficiency virus type 2 (HIV-2) strains vary widely in their abilities to grow in Old World monkey (OWM) cells such as those of cynomolgus monkeys (CM). We evaluated eight HIV-2 isolates for their sensitivities to CM TRIM5{alpha}, an anti-HIV factor in OWM cells. We found that different HIV-2 isolates showed differences in their sensitivities to CM TRIM5{alpha}. Sequence analysis showed that TRIM5{alpha}-sensitive viruses had proline at the 120th position of the capsid protein (CA), whereas TRIM5{alpha}-resistant viruses had either alanine or glutamine. Mutagenesis studies indicated that the single amino acid at the 120th position indeed affected the sensitivity of the virus to CM TRIM5{alpha}.


* Corresponding author. Mailing address: Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-Oka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8346. Fax: 81-6-6879-8347. E-mail: shioda{at}biken.osaka-u.ac.jp

{triangledown} Published ahead of print on 2 May 2007.


Journal of Virology, July 2007, p. 7280-7285, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00406-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2007 by the American Society for Microbiology. All rights reserved.