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Journal of Virology, July 2007, p. 7280-7285, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00406-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Single Amino Acid of the Human Immunodeficiency Virus Type 2 Capsid Affects Its Replication in the Presence of Cynomolgus Monkey and Human TRIM5{alpha}s{triangledown}

Haihan Song,1 Emi E. Nakayama,1 Masaru Yokoyama,2 Hironori Sato,2 Jay A. Levy,3 and Tatsuo Shioda1*

Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan,1 Laboratory of Viral Genomics, Center for Pathogen Genomics, National Institute of Infectious Diseases, Tokyo 208-0011, Japan,2 Cancer Research Institute, School of Medicine, University of California, San Francisco, California 941433

Received 26 February 2007/ Accepted 23 April 2007

Human immunodeficiency virus type 2 (HIV-2) strains vary widely in their abilities to grow in Old World monkey (OWM) cells such as those of cynomolgus monkeys (CM). We evaluated eight HIV-2 isolates for their sensitivities to CM TRIM5{alpha}, an anti-HIV factor in OWM cells. We found that different HIV-2 isolates showed differences in their sensitivities to CM TRIM5{alpha}. Sequence analysis showed that TRIM5{alpha}-sensitive viruses had proline at the 120th position of the capsid protein (CA), whereas TRIM5{alpha}-resistant viruses had either alanine or glutamine. Mutagenesis studies indicated that the single amino acid at the 120th position indeed affected the sensitivity of the virus to CM TRIM5{alpha}.

* Corresponding author. Mailing address: Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-Oka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8346. Fax: 81-6-6879-8347. E-mail: shioda{at}biken.osaka-u.ac.jp

{triangledown} Published ahead of print on 2 May 2007.

Journal of Virology, July 2007, p. 7280-7285, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00406-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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