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Journal of Virology, July 2007, p. 7171-7177, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00200-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Establishment of a Cottontail Rabbit Papillomavirus/HLA-A2.1 Transgenic Rabbit Model{triangledown}

Jiafen Hu,1,2 Xuwen Peng,4 Lynn R. Budgeon,1,2 Nancy M. Cladel,1,2 Karla K. Balogh,1,2 and Neil D. Christensen1,2,3*

Jake Gittlen Cancer Research Foundation,1 Department of Pathology,2 Department of Microbiology and Immunology,3 Department of Comparative Medicine, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 170334

Received 29 January 2007/ Accepted 16 April 2007

Three transgenic rabbit lines that express a well-characterized human major histocompatibility complex class I (MHC-I) gene (HLA-A2.1) have been established. All three lines carry the HLA-A2.1 heavy chain and are able to pass the transgene to their offspring with both the outbred and the inbred EIII/JC genetic background. HLA-A2.1 colocalizes exclusively with rabbit MHC-I on the cell surfaces. These HLA-A2.1 transgenic rabbits demonstrated infection patterns similar to those found after cottontail rabbit papillomavirus (CRPV) challenge when compared with results in normal rabbits, although higher regression rates were found in HLA-A2.1 transgenic rabbits. Because the CRPV genome can accommodate significant modifications, the CRPV/HLA-A2.1 rabbit model has the potential to be used to screen HLA-A2.1-restricted immunogenic epitopes from human papillomaviruses in the context of in vivo papillomavirus infection.

* Corresponding author. Mailing address: Jake Gittlen Cancer Research Foundation, 500 University Dr., Pennsylvania State University, Hershey, PA 17033-2390. Phone: (717) 531-6185. Fax: (717) 531-5634. E-mail: ndc1{at}psu.edu

{triangledown} Published ahead of print on 25 April 2007.

Journal of Virology, July 2007, p. 7171-7177, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00200-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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