This Article Full Text Full Text (PDF) Other Versions of this Article: JVI.00244-07v1 81/13/6993    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Qiu, J. Articles by Pintel, D. Search for Related Content PubMed PubMed Citation Articles by Qiu, J. Articles by Pintel, D.

 Previous Article  |  Next Article 

Journal of Virology, July 2007, p. 6993-7000, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00244-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Abundant R2 mRNA Generated by Aleutian Mink Disease Parvovirus Is Tricistronic, Encoding NS2, VP1, and VP2

Jianming Qiu, Fang Cheng, and David Pintel^*

Department of Molecular Microbiology and Immunology, University of Missouri—Columbia, School of Medicine, Life Sciences Center, Columbia, Missouri 65212

Received 5 February 2007/ Accepted 5 April 2007

The abundant R2 mRNA encoded by the single left-end promoter of Aleutian mink disease parvovirus is tricistronic; it not only expresses the capsid proteins VP1 and VP2 but is also the major source for the nonstructural protein NS2. A cis-acting sequence within the NS2 gene was shown to be required for efficient capsid protein production, and its effect displayed a distinct location dependence. Ribosome transit through the upstream NS2 gene region was necessary for efficient VP1 and VP2 expression; however, neither ablation nor improvement of the NS2 initiating AUG had an effect on capsid protein production, suggesting that the translation of the NS2 protein per se had little influence on VP1 and VP2 expression. Thus, proper control of the alternative translation of the tricistronic R2 mRNA, a process critical for viral replication, is governed in a complex manner.

Published ahead of print on 11 April 2007.

Present address: Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160.