This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ekman, A.
Right arrow Articles by Söderlund-Venermo, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ekman, A.
Right arrow Articles by Söderlund-Venermo, M.

 Previous Article  |  Next Article 

Journal of Virology, July 2007, p. 6927-6935, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02713-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3{triangledown}

Anna Ekman,1,{dagger},{ddagger} Kati Hokynar,1*,{ddagger} Laura Kakkola,1 Kalle Kantola,1 Lea Hedman,1 Heidi Bondén,1 Matthias Gessner,2 Claudia Aberham,2 Päivi Norja,1 Simo Miettinen,1 Klaus Hedman,1 and Maria Söderlund-Venermo1

Department of Virology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital Laboratory, Helsinki, Finland,1 Plasma Analytics, Baxter AG, Vienna, Austria2

Received 8 December 2006/ Accepted 26 March 2007

The human parvovirus B19 is now divided into three genotypes: type 1 (prototype), type 2 (A6- and LaLi-like), and type 3 (V9-like). In overall DNA sequence, the three virus types differ by ~10%. The most striking DNA dissimilarity, of >20%, is observed within the p6 promoter region. Because of the scarcity of data on the biological activities and pathogenetic potentials of virus types 2 and 3, we examined the functional characteristics of these virus types. We found the activities of the three p6 promoters to be of equal strength and to be most active in B19-permissive cells. Virus type 2 capsid protein VP2, alone or together with VP1, was expressed with the baculovirus system and was shown to assemble into icosahedral parvovirus-like particles, which were reactive in the hemagglutination assay. Furthermore, sera containing DNA of any of the three B19 types were shown to hemagglutinate. The infectivities of these sera were examined in two B19-permissive cell lines. Reverse transcription-PCR revealed synthesis of spliced B19 mRNAs, and immunofluorescence verified the production of NS and VP proteins in the infected cells. All three genotypes showed similar functional characteristics in all experiments performed, showing that the three virus types indeed belong to the same species, i.e., human parvovirus B19. Additionally, the antibody activity in sera from B19 type 1- or type 2-infected subjects (long-term immunity) was examined with homo- and heterologous virus-like particles. Cross-reactivity of 100% was observed, indicating that the two B19 genotypes comprise a single serotype.

* Corresponding author. Mailing address: Department of Virology, Haartman Institute, P.O. Box 21 (Haartmaninkatu 3), University of Helsinki, Helsinki, Finland. Phone: 358-9-19126676. Fax: 358-9-19126491. E-mail: Kati.Hokynar{at}helsinki.fi

{triangledown} Published ahead of print on 4 April 2007.

{dagger} Present address: Division of Anatomy, Department of Basic Veterinary Sciences, University of Helsinki, Helsinki, Finland.

{ddagger} A. Ekman and K. Hokynar contributed equally to this study.

Journal of Virology, July 2007, p. 6927-6935, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02713-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guan, W., Wong, S., Zhi, N., Qiu, J. (2009). The Genome of Human Parvovirus B19 Can Replicate in Nonpermissive Cells with the Help of Adenovirus Genes and Produces Infectious Virus. J. Virol. 83: 9541-9553 [Abstract] [Full Text]  
  • Pillet, S., Fichelson, S., Morinet, F., Young, N. S., Zhi, N., Wong, S. (2008). Human B19 Erythrovirus In Vitro Replication: What's New?. J. Virol. 82: 8951-8953 [Full Text]  
  • Norja, P., Eis-Hubinger, A. M., Soderlund-Venermo, M., Hedman, K., Simmonds, P. (2008). Rapid Sequence Change and Geographical Spread of Human Parvovirus B19: Comparison of B19 Virus Evolution in Acute and Persistent Infections. J. Virol. 82: 6427-6433 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»