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Journal of Virology, July 2007, p. 6858-6868, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02472-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Activated CD34-Derived Langerhans Cells Mediate Transinfection with Human Immunodeficiency Virus{triangledown}

Kelly M. Fahrbach,1,{dagger} Sheila M. Barry,1,2,{dagger} Seyoum Ayehunie,3 Sarah Lamore,3 Mitchell Klausner,3 and Thomas J. Hope1*

Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611,1 Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612,2 MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 017213

Received 9 November 2006/ Accepted 10 April 2007

Langerhans cells (LCs) are a subset of dendritic cells (DCs) that reside within epidermal and mucosal tissue. Because of their location, LCs are potentially the first cells to encounter human immunodeficiency virus (HIV) during sexual transmission. We report that LCs purified from CD34+-derived DCs can facilitate the transinfection of target cells but only after activation. Virions were observed in an intracellular compartment that contains several tetraspanins, in addition to the unique LC markers langerin and CD1a. This reveals that the trafficking of HIV within LCs is reminiscent of that which occurs in mature monocyte-derived DCs and that it varies with the activation state of the cell. The observation that activated LCs can mediate transinfection suggests a potential role for these cells in the known increase in HIV transmission associated with sexually transmitted infections that would cause inflammation of the genital lining.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Ward 8-140, 303 E. Chicago Avenue, Chicago, IL 60611-3008. Phone: (312) 503-1360. Fax: (312) 503-2696. E-mail: thope{at}northwestern.edu

{triangledown} Published ahead of print on 18 April 2007.

{dagger} These authors contributed equally.

Journal of Virology, July 2007, p. 6858-6868, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02472-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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