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Journal of Virology, July 2007, p. 6807-6816, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00338-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Interaction of the C-Terminal Domains of Sendai Virus N and P Proteins: Comparison of Polymerase-Nucleocapsid Interactions within the Paramyxovirus Family{triangledown}

Klaartje Houben,1 Dominique Marion,1 Nicolas Tarbouriech,2 Rob W. H. Ruigrok,2 and Laurence Blanchard1*

Institut de Biologie Structurale "Jean-Pierre Ebel," UMR 5075, CEA-CNRS-UJF, 41 Rue Jules Horowitz, 38027 Grenoble Cedex 1, France,1 Unit for Virus Host Cell Interactions, UMR 5233 UJF-EMBL-CNRS, Boite Postale 181, 38042 Grenoble Cedex 9, France2

Received 15 February 2007/ Accepted 12 April 2007

Interaction of the C-terminal domains of Sendai virus (SeV) P and N proteins is crucial for RNA synthesis by correctly positioning the polymerase complex (L+P) onto the nucleocapsid (N/RNA). To better understand this mechanism within the paramyxovirus family, we have studied the complex formed by the SeV C-terminal domains of P (PX) and N (NTAIL) proteins by solution nuclear magnetic resonance spectroscopy. We have characterized SeV NTAIL, which belongs to the class of intrinsically disordered proteins, and precisely defined the binding regions within this latter domain and within PX. SeV NTAIL binds with residues 472 to 493, which have a helical propensity (residues 477 to 491) to the surface created by helices {alpha}2 and {alpha}3 of PX with a 1:1 stoichiometry, as was also found for measles virus (MV). The binding interface is dominated by charged residues, and the dissociation constant was determined to be 57 ± 18 µM under conditions of the experiment (i.e., in 0.5 M NaCl). We have also shown that the extreme C terminus of SeV NTAIL does not interact with PX, which is in contrast to MV, where a second binding site was identified. In addition, the interaction surfaces of the MV proteins are hydrophobic and a stronger binding constant was found. This gives a good illustration of how selection pressure allowed the C-terminal domains of N and P proteins to evolve concomitantly within this family of viruses in order to lead to protein complexes having the same three-dimensional fold, and thus the same function, but with completely different binding interfaces.

* Corresponding author. Mailing address: CEA Cadarache, IBEB, LEMiRE, Saint-Paul-lez-Durance, F-13108 France. Phone: 33 4 42 25 7863. Fax: 33 4 42 25 6648. E-mail: Laurence.Blanchard{at}cea.fr

{triangledown} Published ahead of print on 25 April 2007.

Journal of Virology, July 2007, p. 6807-6816, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.00338-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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