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Journal of Virology, June 2007, p. 6765-6770, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.02579-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Mutagenesis of the Herpesvirus Saimiri Terminal Repeat Region Reveals Important Elements for Virus Production{triangledown}

Robert E. White,* Lindsay Carline, and Martin J. Allday

Department of Virology, Faculty of Medicine, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom

Received 22 November 2006/ Accepted 2 April 2007

Deletion of the terminal repeats (TR) from herpesvirus saimiri (HVS) renders it unable to produce infectious virus or generate plaques. However, a TR-deleted HVS bacterial artificial chromosome can form replication compartments. Complementation of this mutant shows that one copy of the TR, plus the right junction of the genome with the TR, is sufficient for efficient plaque formation and generation of infectious virus. Within the TR unit, the region around the cleavage site of the genome appears both necessary and sufficient for virus production. Analysis of episomes from productive cells indicates a propensity to amplify TR numbers during the lytic cycle.

* Corresponding author. Mailing address: Department of Virology, Faculty of Medicine, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom. Phone: (44) 207 594 3838. Fax: (44) 207 594 3973. E-mail: robert.e.white{at}imperial.ac.uk

{triangledown} Published ahead of print on 11 April 2007.

Journal of Virology, June 2007, p. 6765-6770, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.02579-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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