This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, E.
Right arrow Articles by Sun, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, E.
Right arrow Articles by Sun, R.

 Previous Article  |  Next Article 

Journal of Virology, June 2007, p. 6761-6764, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.02726-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Murine Gammaherpesvirus 68 Open Reading Frame 24 Is Required for Late Gene Expression after DNA Replication{triangledown}

Elaine Wong,1 Ting-Ting Wu,1 Nichole Reyes,1 Hongyu Deng,2,3 and Ren Sun1,2*

Department of Molecular and Medical Pharmacology,1 Dental Research Institute, University of California, Los Angeles, California 90095,2 Center for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, People's Republic of China3

Received 11 December 2006/ Accepted 19 March 2007

Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown to be defective in late gene expression. Expression of early genes, as well as viral genome replication, was not affected. Furthermore, the defect in late gene expression was likely due to a deficiency in transcription. Thus, we have identified an MHV-68 protein, ORF24, that is essential for the expression of viral late proteins yet dispensable for viral DNA replication.

* Corresponding author. Mailing address: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 23-120 Center for Health Sciences, Los Angeles, CA 90095-1735. Phone: (310) 794-5557. Fax: (310) 825-6267. E-mail: rsun{at}mednet.ucla.edu

{triangledown} Published ahead of print on 28 March 2007.

Journal of Virology, June 2007, p. 6761-6764, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.02726-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wu, T.-T., Park, T., Kim, H., Tran, T., Tong, L., Martinez-Guzman, D., Reyes, N., Deng, H., Sun, R. (2009). ORF30 and ORF34 Are Essential for Expression of Late Genes in Murine Gammaherpesvirus 68. J. Virol. 83: 2265-2273 [Abstract] [Full Text]  
  • Arumugaswami, V., Sitapara, R., Hwang, S., Song, M. J., Ho, T. N., Su, N. Q., Sue, E. Y., Kanagavel, V., Xing, F., Zhang, X., Zhao, M., Deng, H., Wu, T.-T., Kanagavel, S., Zhang, L., Dandekar, S., Papp, J., Sun, R. (2009). High-Resolution Functional Profiling of a Gammaherpesvirus RTA Locus in the Context of the Viral Genome. J. Virol. 83: 1811-1822 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»