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Journal of Virology, June 2007, p. 6294-6306, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.02648-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Transcriptional Repressor K-RBP Modulates RTA-Mediated Transactivation and Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus{triangledown}

Zhilong Yang and Charles Wood*

Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588

Received 30 November 2006/ Accepted 26 March 2007

The replication and transcription activator (RTA) protein of Kaposi's sarcoma (KS)-associated herpesvirus (KSHV)/human herpesvirus 8 functions as the key regulator to induce KSHV lytic replication from latency through activation of the lytic cascade of KSHV. Elucidation of the host factors involved in RTA-mediated transcriptional activation is pivotal for understanding the transition between viral latency and lytic replication. KSHV-RTA binding protein (K-RBP) was previously isolated as a cellular RTA binding protein of unknown function. Sequence analysis showed that K-RBP contains a Kruppel-associated box (KRAB) at the N terminus and 12 adjacent zinc finger motifs. In similarity to other KRAB-containing zinc finger proteins, K-RBP is a transcriptional repressor. Mutational analysis revealed that the KRAB domain is responsible for the transcriptional suppression activity of this protein and that the repression is histone deacetylase independent. K-RBP was found to repress RTA-mediated transactivation and interact with TIF1ß (transcription intermediary factor 1ß), a common corepressor of KRAB-containing protein, to synergize with K-RBP in repression. Overexpression and knockdown experiment results suggest that K-RBP is a suppressor of RTA-mediated KSHV reactivation. Our findings suggest that the KRAB-containing zinc finger protein K-RBP can suppress RTA-mediated transactivation and KSHV lytic replication and that KSHV utilizes this protein as a regulator to maintain a balance between latency and lytic replication.

* Corresponding author. Mailing address: Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, E249 Beadle Center, P.O. Box 880666, Lincoln, NE 68588-0666. Phone: (402) 472-4550. Fax: (402) 472-8722. E-mail: cwood1{at}unl.edu

{triangledown} Published ahead of print on 4 April 2007.

Journal of Virology, June 2007, p. 6294-6306, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.02648-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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