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Journal of Virology, June 2007, p. 6151-6155, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.00414-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Development of a Cell-Based Assay Probing the Specific Interaction between the Human Immunodeficiency Virus Type 1 Nucleocapsid and Psi RNA In Vivo{triangledown}

Soo In Jang,1 Young Ho Kim,1 Soon Young Paik,2 and Ji Chang You1,3*

National Research Laboratory of Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea,1 Department of Microbiology, College of Medicine, The Catholic University of Korea,2 Avixgen Inc., Seocho-gu Banpo-dong 505, Seoul 137-701, Korea3

Received 27 February 2007/ Accepted 5 March 2007

Here, we describe a cell-based in vivo assay that probes the specific interaction between nucleocapsid (NC) protein and Psi ({Psi}) RNA, the human immunodeficiency virus (HIV) packaging signal. The results demonstrate for the first time a specific NC-{Psi} interaction within living cells. The specificity and applicability of the assay were confirmed by mutational studies of NC and deletion-mapping analyses of {Psi}-RNA as well as by testing the in vivo NC-binding effects of NC-aptamer RNAs identified previously in vitro. This assay system would facilitate further detailed studies of the NC-{Psi} interaction in vivo and the screening of various anti-HIV molecules targeting NC and the specific interaction.

* Corresponding author. Mailing address: Department of Pathology, College of Medicine, The Catholic University of Korea, Seocho-gu Banpo-dong 505, Seoul 137-701, Korea. Phone: 82-2-590-1318. Fax: 82-2-595-2241. E-mail: jiyou{at}catholic.ac.kr

{triangledown} Published ahead of print on 14 March 2007.

Journal of Virology, June 2007, p. 6151-6155, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.00414-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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