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Journal of Virology, June 2007, p. 6106-6110, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.00070-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sodium Butyrate: a Chemical Inducer of In Vivo Reactivation of Herpes Simplex Virus Type 1 in the Ocular Mouse Model{triangledown}

Donna M. Neumann,1 Partha S. Bhattacharjee,1 and James M. Hill1,2,3*

Department of Ophthalmology (LSU Eye Center of Excellence),1 LSUHSC Neuroscience Center,2 Departments of Pharmacology and Microbiology, Louisiana State University Health Sciences Center at New Orleans, New Orleans, Louisiana3

Received 10 January 2007/ Accepted 2 March 2007

Recent studies have explored the chromatin structures associated with the herpes simplex virus type 1 (HSV-1) genome during latency, particularly with regard to specific histone tail modifications such as acetylation and dimethylation. The objective of our present study was to develop a rapid systemic method of in vivo HSV-1 reactivation to further explore the changes that occur in the chromatin structures associated with HSV-1 at early time points after the initiation of HSV reactivation. We present a uniform, rapid, and reliable method of in vivo HSV-1 reactivation in mice that yields high reactivation frequencies (75 to 100%) by using sodium butyrate, a histone deacetylase inhibitor, and demonstrate that the reactivating virus can be detected at the original site of infection.

* Corresponding author. Mailing address: LSU Eye Center, 2020 Gravier St., Suite B, New Orleans, LA 70112. Phone: (504) 568-2274. Fax: (504) 568-2385. E-mail: jhill{at}lsuhsc.edu

{triangledown} Published ahead of print on 14 March 2007.

Journal of Virology, June 2007, p. 6106-6110, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.00070-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Whitlow, Z. W., Kristie, T. M. (2009). Recruitment of the Transcriptional Coactivator HCF-1 to Viral Immediate-Early Promoters during Initiation of Reactivation from Latency of Herpes Simplex Virus Type 1. J. Virol. 83: 9591-9595 [Abstract] [Full Text]  
  • Preston, C. M., Nicholl, M. J. (2008). Induction of Cellular Stress Overcomes the Requirement of Herpes Simplex Virus Type 1 for Immediate-Early Protein ICP0 and Reactivates Expression from Quiescent Viral Genomes. J. Virol. 82: 11775-11783 [Abstract] [Full Text]  
  • Neumann, D. M., Bhattacharjee, P. S., Giordani, N. V., Bloom, D. C., Hill, J. M. (2007). In Vivo Changes in the Patterns of Chromatin Structure Associated with the Latent Herpes Simplex Virus Type 1 Genome in Mouse Trigeminal Ganglia Can Be Detected at Early Times after Butyrate Treatment. J. Virol. 81: 13248-13253 [Abstract] [Full Text]  


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