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Journal of Virology, May 2007, p. 5413-5417, Vol. 81, No. 10
0022-538X/07/$08.00+0     doi:10.1128/JVI.02554-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Independent Evolution of Human Immunodeficiency Virus Type 1 env V1/V2 and V4/V5 Hypervariable Regions during Chronic Infection{triangledown}

Patrick R. Harrington,1,{dagger} Julie A. E. Nelson,2 Kathryn M. Kitrinos,2,3,{ddagger} and Ronald Swanstrom1,2,4*

Lineberger Comprehensive Cancer Center,1 UNC Center for AIDS Research,2 Curriculum in Genetics and Molecular Biology,3 Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599-72954

Received 20 November 2006/ Accepted 20 February 2007

Using DNA heteroduplex tracking assays, we characterized human immunodeficiency virus type 1 env V4/V5 genetic populations in multiple blood plasma samples collected over an average of 7 months from 24 chronically infected human subjects. We observed complex and dynamic V4/V5 genetic populations in most subjects. Comparisons of V4/V5 and V1/V2 population changes over the course of the study showed that major shifts in genetic populations frequently occurred in one region but not the other, and these observations were independently confirmed in one subject by single-genome sequencing. These results suggest that the V1/V2 and V4/V5 regions of env often evolve independently during chronic infection.

* Corresponding author. Mailing address: University of North Carolina at Chapel Hill, 22-062 Lineberger Cancer Center, CB#7295, Chapel Hill, NC 27599-7295. Phone: (919) 966-5710. Fax: (919) 966-8212. E-mail: risunc{at}med.unc.edu

{triangledown} Published ahead of print on 28 February 2007.

{dagger} Present address: Carolina Vaccine Institute, University of North Carolina, Chapel Hill, NC 27599-7292.

{ddagger} Present address: International Clinical Virology, GlaxoSmithKline, P.O. Box 13398, 5 Moore Dr., RTP, NC 27709-3398.

Journal of Virology, May 2007, p. 5413-5417, Vol. 81, No. 10
0022-538X/07/$08.00+0     doi:10.1128/JVI.02554-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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