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Journal of Virology, April 2006, p. 4147-4156, Vol. 80, No. 8
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.8.4147-4156.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cell Cycle Perturbations Induced by Infection with the Coronavirus Infectious Bronchitis Virus and Their Effect on Virus Replication

Brian Dove,¹ Gavin Brooks,² Katrina Bicknell,² Torsten Wurm,^3, and Julian A. Hiscox^1,4*

Institute of Molecular and Cellular Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom,¹ School of Pharmacy, University of Reading, Reading RG6 6AJ, United Kingdom,² School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, United Kingdom,³ Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom⁴

Received 6 October 2005/ Accepted 2 February 2006

In eukaryotic cells, cell growth and division occur in a stepwise, orderly fashion described by a process known as the cell cycle. The relationship between positive-strand RNA viruses and the cell cycle and the concomitant effects on virus replication are not clearly understood. We have shown that infection of asynchronously replicating and synchronized replicating cells with the avian coronavirus infectious bronchitis virus (IBV), a positive-strand RNA virus, resulted in the accumulation of infected cells in the G₂/M phase of the cell cycle. Analysis of various cell cycle-regulatory proteins and cellular morphology indicated that there was a down-regulation of cyclins D1 and D2 (G₁ regulatory cyclins) and that a proportion of virus-infected cells underwent aberrant cytokinesis, in which the cells underwent nuclear, but not cytoplasmic, division. We assessed the impact of the perturbations on the cell cycle for virus-infected cells and found that IBV-infected G₂/M-phase-synchronized cells exhibited increased viral protein production when released from the block when compared to cells synchronized in the G₀ phase or asynchronously replicating cells. Our data suggested that IBV induces a G₂/M phase arrest in infected cells to promote favorable conditions for viral replication.

Present address: Institute for Molecular Virology and McArdle Cancer Research Facility, University of Wisconsin-Madison, Madison, Wis.

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