This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, J.-S.
Right arrow Articles by Kim, C.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, J.-S.
Right arrow Articles by Kim, C.-J.
Right arrowPubmed/NCBI databases
*Substance via MeSH

 Previous Article  |  Next Article 

Journal of Virology, April 2006, p. 4079-4087, Vol. 80, No. 8
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.8.4079-4087.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mucosal Immunization with Surface-Displayed Severe Acute Respiratory Syndrome Coronavirus Spike Protein on Lactobacillus casei Induces Neutralizing Antibodies in Mice

Jong-Soo Lee,1,{dagger} Haryoung Poo,2,{dagger} Dong P. Han,3 Seung-Pyo Hong,1 Kwang Kim,1 Michael W. Cho,3 Eun Kim,5 Moon-Hee Sung,1,4* and Chul-Joong Kim5*

Bioleaders Corporation, Daejeon, Korea,1 Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea;,2 Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio,3 Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University, Seoul, Korea,4 National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University, Daejeon, Korea5

Received 27 July 2005/ Accepted 16 January 2006

Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-{gamma}-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.

* Corresponding author. Mailing address for M.-H. Sung: Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University, Seoul, Korea. Phone: 82-2-910-8550. Fax: 82-2-910-5098. E-mail: smoonhee{at}kookmin.ac.kr. Mailing address for C.-J. Kim: National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University, Daejeon, Korea. Phone: 82-42-821-6783. Fax: 82-42-823-9382. E-mail: cjkim{at}cnu.ac.kr.

{dagger} Jong-Soo Lee and Haryoung Poo contributed equally to this work.

Journal of Virology, April 2006, p. 4079-4087, Vol. 80, No. 8
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.8.4079-4087.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, J.-K., Hou, X.-L., Wei, C.-H., Yu, L.-Y., He, X.-J., Wang, G.-H., Lee, J.-S., Kim, C.-J. (2009). Induction of Immune Responses in Mice after Oral Immunization with Recombinant Lactobacillus casei Strains Expressing Enterotoxigenic Escherichia coli F41 Fimbrial Protein. Appl. Environ. Microbiol. 75: 4491-4497 [Abstract] [Full Text]  
  • Kajikawa, A., Igimi, S. (2009). Reduction of Tumor Necrosis Factor Alpha-Inducing Capacity of Recombinant Lactobacillus casei via Expression of Salmonella OmpC. Appl. Environ. Microbiol. 75: 2727-2734 [Abstract] [Full Text]  
  • del Rio, B., Dattwyler, R. J., Aroso, M., Neves, V., Meirelles, L., Seegers, J. F. M. L., Gomes-Solecki, M. (2008). Oral Immunization with Recombinant Lactobacillus plantarum Induces a Protective Immune Response in Mice with Lyme Disease. CVI 15: 1429-1435 [Abstract] [Full Text]  
  • Du, L., Zhao, G., Lin, Y., Sui, H., Chan, C., Ma, S., He, Y., Jiang, S., Wu, C., Yuen, K.-Y., Jin, D.-Y., Zhou, Y., Zheng, B.-J. (2008). Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection. J. Immunol. 180: 948-956 [Abstract] [Full Text]  
  • Han, D. P., Lohani, M., Cho, M. W. (2007). Specific Asparagine-Linked Glycosylation Sites Are Critical for DC-SIGN- and L-SIGN-Mediated Severe Acute Respiratory Syndrome Coronavirus Entry. J. Virol. 81: 12029-12039 [Abstract] [Full Text]  
  • Luo, F., Feng, Y., Liu, M., Li, P., Pan, Q., Jeza, V. T., Xia, B., Wu, J., Zhang, X.-L. (2007). Type IVB Pilus Operon Promoter Controlling Expression of the Severe Acute Respiratory Syndrome-Associated Coronavirus Nucleocapsid Gene in Salmonella enterica Serovar Typhi Elicits Full Immune Response by Intranasal Vaccination. CVI 14: 990-997 [Abstract] [Full Text]  
  • Rockx, B., Sheahan, T., Donaldson, E., Harkema, J., Sims, A., Heise, M., Pickles, R., Cameron, M., Kelvin, D., Baric, R. (2007). Synthetic Reconstruction of Zoonotic and Early Human Severe Acute Respiratory Syndrome Coronavirus Isolates That Produce Fatal Disease in Aged Mice. J. Virol. 81: 7410-7423 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»