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Journal of Virology, April 2006, p. 3655-3659, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3655-3659.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Bioluminescence Imaging of Live Infected Salmonids Reveals that the Fin Bases Are the Major Portal of Entry for Novirhabdovirus

Abdallah Harmache,1 Monique LeBerre,1 Stéphanie Droineau,1 Marco Giovannini,2 and Michel Brémont1*

Unité de Virologie et Immunologie Moléculaires, INRA, CRJ Domaine de Vilvert, 78352 Jouy en Josas, France,1 Inserm U434 Fondation Jean Dausset, CEPH 27 rue Juliette Dodu, 75010 Paris, France2

Received 10 November 2005/ Accepted 13 January 2006

Although Novirhabdovirus viruses, like the Infectious hematopietic necrosis virus (IHNV), have been extensively studied, limited knowledge exists on the route of IHNV entry during natural infection. A recombinant IHNV (rIHNV) expressing the Renilla luciferase gene was generated and used to infect trout. A noninvasive bioluminescence assay was developed so that virus replication in live fish could be followed hours after infection. We provide here evidence that the fin bases are the portal of entry into the fish. Confirmation was brought by the use of a nonpathogenic rIHNV, which was shown to persist in fins for 3 weeks postinfection.

* Corresponding author. Mailing address: Unité de Virologie et Immunologie Moléculaires, INRA, CRJ Domaine de Vilvert, 78352 Jouy en Josas, France. Phone: 33 (1) 34 65 26 15. Fax: 33 (1) 34 65 26 21. E-mail: michel.bremont{at}jouy.inra.fr.

Journal of Virology, April 2006, p. 3655-3659, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3655-3659.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.