This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johne, R.
Right arrow Articles by Müller, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johne, R.
Right arrow Articles by Müller, H.

 Previous Article  |  Next Article 

Journal of Virology, April 2006, p. 3523-3531, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3523-3531.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes

Reimar Johne,1* Walter Wittig,2 Daniel Fernández-de-Luco,3 Ursula Höfle,4 and Hermann Müller1

Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany,1 Dresden, Germany,2 Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Zaragoza, Spain,3 Centro de Investigación Agropecuaria, El Deheson del Encinar, Oropesa, Spain4

Received 7 October 2005/ Accepted 13 January 2006

Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds—avian polyomavirus (APV) and goose hemorrhagic polyomavirus (GHPV)—are the primary agents of acute and chronic disease with high mortality rates in young birds. Screening of field samples of diseased birds by consensus PCR revealed the presence of two novel polyomaviruses in the liver of an Eurasian bullfinch (Pyrrhula pyrrhula griseiventris) and in the spleen of a Eurasian jackdaw (Corvus monedula), tentatively designated as finch polyomavirus (FPyV) and crow polyomavirus (CPyV), respectively. The genomes of the viruses were amplified by using multiply primed rolling-circle amplification and cloned. Analysis of the FPyV and CPyV genome sequences revealed a close relationship to APV and GHPV, indicating the existence of a distinct avian group among the polyomaviruses. The main characteristics of this group are (i) involvement in fatal disease, (ii) the existence of an additional open reading frame in the 5' region of the late mRNAs, and (iii) a different manner of DNA binding of the large tumor antigen compared to that of the mammalian polyomaviruses.

* Corresponding author. Mailing address: Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany. Phone: 49 341 9738204. Fax: 49 341 9738219. E-mail: johne{at}vetmed.uni-leipzig.de.

Journal of Virology, April 2006, p. 3523-3531, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3523-3531.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Misra, V., Dumonceaux, T., Dubois, J., Willis, C., Nadin-Davis, S., Severini, A., Wandeler, A., Lindsay, R., Artsob, H. (2009). Detection of polyoma and corona viruses in bats of Canada. J. Gen. Virol. 90: 2015-2022 [Abstract] [Full Text]  
  • Kim, K.-H., Chang, H.-W., Nam, Y.-D., Roh, S. W., Kim, M.-S., Sung, Y., Jeon, C. O., Oh, H.-M., Bae, J.-W. (2008). Amplification of Uncultured Single-Stranded DNA Viruses from Rice Paddy Soil. Appl. Environ. Microbiol. 74: 5975-5985 [Abstract] [Full Text]  
  • Woolford, L., Rector, A., Van Ranst, M., Ducki, A., Bennett, M. D., Nicholls, P. K., Warren, K. S., Swan, R. A., Wilcox, G. E., O'Hara, A. J. (2007). A Novel Virus Detected in Papillomas and Carcinomas of the Endangered Western Barred Bandicoot (Perameles bougainville) Exhibits Genomic Features of both the Papillomaviridae and Polyomaviridae. J. Virol. 81: 13280-13290 [Abstract] [Full Text]  
  • Johne, R., Muller, H. (2007). Polyomaviruses of Birds: Etiologic Agents of Inflammatory Diseases in a Tumor Virus Family. J. Virol. 81: 11554-11559 [Full Text]  
  • Allander, T., Andreasson, K., Gupta, S., Bjerkner, A., Bogdanovic, G., Persson, M. A. A., Dalianis, T., Ramqvist, T., Andersson, B. (2007). Identification of a Third Human Polyomavirus. J. Virol. 81: 4130-4136 [Abstract] [Full Text]  
  • Johne, R., Paul, G., Enderlein, D., Stahl, T., Grund, C., Muller, H. (2007). Avian polyomavirus mutants with deletions in the VP4-encoding region show deficiencies in capsid assembly and virus release, and have reduced infectivity in chicken. J. Gen. Virol. 88: 823-830 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»