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Journal of Virology, April 2006, p. 3406-3415, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3406-3415.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Human Chromosome 2 Carries a Gene Required for Production of Infectious Human Immunodeficiency Virus Type 1

Ayse K. Coskun,1 Marc van Maanen,1 Van Nguyen,1 and Richard E. Sutton1,2*

Department of Molecular Virology and Microbiology,1 Center for Cell and Gene Therapy and Department of Medicine, Division of Infectious Diseases, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 770302

Received 3 November 2005/ Accepted 16 January 2006

Human immunodeficiency virus type 1 (HIV) replicates only in certain primate cells. In murine cells expressing cyclin T1, a posttranscriptional block exists such that small amounts of capsid and little infectious virus are released. This block is relieved in part by fusion with human cells. Here we have tested a panel of mouse-human somatic cell hybrids for production of infectious virus. Only those containing human chromosome 2 were permissive, which correlated with capsid production. The effect was specific to HIV in that release of murine leukemia virus was minimally affected by the presence of chromosome 2. Although expression of Vpu markedly increased capsid production in the absence of chromosome 2, it did not result in a corresponding increase in infectious HIV. The presence of chromosome 2 did not have consistent effects on the amount of unspliced viral RNA, whereas the amount of cell-associated Gag p55 was increased a fewfold. These results suggest that processing of HIV Gag can be corrected by one or more genes present on human chromosome 2 to allow production of infectious HIV from murine cells.

* Corresponding author. Mailing address: Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Rm. 917D, Houston, TX 77030. Phone: (713) 798-4096. Fax: (713) 798-3586. E-mail: rsutton{at}bcm.tmc.edu.

Journal of Virology, April 2006, p. 3406-3415, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3406-3415.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wei, M., Yang, Y., Niu, M., Desfosse, L., Kennedy, R., Musier-Forsyth, K., Kleiman, L. (2008). Inability of Human Immunodeficiency Virus Type 1 Produced in Murine Cells To Selectively Incorporate Primer Formula. J. Virol. 82: 12049-12059 [Abstract] [Full Text]  


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