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Functional Characterization of Heptad Repeat 1 and 2 Mutants of the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus

Institute of Biomedical Sciences, Academia Sinica, Taipei 11529,¹ Division of Biotechnology, Animal Technology Institute Taiwan, Miaoli 35099,² Department of Microbiology, National Taiwan University College of Medicine, Taipei 10051,³ Department of Medical Research, Mackay Memorial Hospital, Taipei County 25115, Taiwan, Republic of China⁴

Received 24 October 2005/ Accepted 13 January 2006

To understand the roles of heptad repeat 1(HR1) and HR2 of the spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) in virus-cell interactions, the conserved Leu or Ile residues located at positions 913, 927, 941, and 955 in HR1 and 1151, 1165, and 1179 in HR2 were individually replaced with an -helix-breaker Pro residue. The 913P mutant was expressed mainly as a faster-migrating, lower-molecular-weight S_L form, while the wild type and all other mutants produced similar levels of both the S_L form and the slower-migrating, higher-molecular-weight S_H form. The wild-type S_L form was processed to the S_H form, whereas the S_L form of the 913P mutant was inefficiently converted to the S_H form after biosynthesis. None of these mutations affected cell surface expression or binding to its cognate ACE2 receptor. In a human immunodeficiency virus type 1/SARS S coexpression study, all mutants except the 913P mutant incorporated the S_H form into the virions as effectively as did the wild-type S_H form. The mutation at Ile-1151 did not affect membrane fusion or viral entry. The impaired viral entry of the 927P, 941P, 955P, and 1165P mutants was due to their inability to mediate membrane fusion, whereas the defect in viral entry of the 1179P mutant occurred not at the stage of membrane fusion but rather at a postfusion stage. Our study demonstrates the functional importance of HR1 and HR2 of the SARS-CoV spike protein in membrane fusion and viral entry.

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