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Journal of Virology, March 2006, p. 3108-3111, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.3108-3111.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Mutations Causing Temperature-Sensitive Defects in Semliki Forest Virus RNA Synthesis

Valeria Lulla,1,2 Andres Merits,2 Peter Sarin,1 Leevi Kääriäinen,1 Sirkka Keränen,1 and Tero Ahola1*

Program in Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland,1 Estonian Biocentre and Institute of Molecular and Cellular Biology, Tartu, Estonia2

Received 7 September 2005/ Accepted 21 December 2005

We have sequenced the nonstructural protein coding region of Semliki Forest virus temperature-sensitive (ts) mutant strains ts1, ts6, ts9, ts10, ts11, ts13, and ts14. In each case, the individual amino acid changes uncovered were transferred to the prototype strain background and thereby identified as the underlying cause of the altered RNA synthesis phenotype. All mutations mapping to the protease domain of nonstructural protein nsP2 caused defects in nonstructural polyprotein processing and subgenomic RNA synthesis, and all mutations in the helicase domain of nsP2 affected subgenomic RNA production. These types of defects were not associated with mutations in other nonstructural proteins.

* Corresponding author. Mailing address: Institute of Biotechnology, P.O. Box 56 (Viikinkaari 9), University of Helsinki, 00014 Helsinki, Finland. Phone: 358-9-19159403. Fax: 358-9-19159560. E-mail: tero.ahola{at}helsinki.fi.

Journal of Virology, March 2006, p. 3108-3111, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.3108-3111.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lulla, V., Sawicki, D. L., Sawicki, S. G., Lulla, A., Merits, A., Ahola, T. (2008). Molecular Defects Caused by Temperature-Sensitive Mutations in Semliki Forest Virus nsP1. J. Virol. 82: 9236-9244 [Abstract] [Full Text]  
  • Kiiver, K., Tagen, I., Zusinaite, E., Tamberg, N., Fazakerley, J. K., Merits, A. (2008). Properties of non-structural protein 1 of Semliki Forest virus and its interference with virus replication. J. Gen. Virol. 89: 1457-1466 [Abstract] [Full Text]  
  • Balistreri, G., Caldentey, J., Kaariainen, L., Ahola, T. (2007). Enzymatic Defects of the nsP2 Proteins of Semliki Forest Virus Temperature-Sensitive Mutants. J. Virol. 81: 2849-2860 [Abstract] [Full Text]  


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