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Journal of Virology, March 2006, p. 3098-3103, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.3098-3103.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rhesus Rhadinovirus R15 Encodes a Functional Homologue of Human CD200

Carly L. Langlais,1,3 John M. Jones,1,3 Ryan D. Estep,1,3 and Scott W. Wong1,2,3*

Vaccine and Gene Therapy Institute, Oregon Health & Science University, West Campus,1 Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon 97006,2 Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon 972013

Received 12 August 2005/ Accepted 28 December 2005

A viral CD200 homologue (vCD200) encoded by open reading frame R15 of rhesus rhadinovirus (RRV), a gammaherpesvirus closely related to human herpesvirus 8 (HHV-8), is described here. RRV vCD200 shares 30% and 28% amino acid identity with human CD200 (huCD200) and HHV-8 vCD200, respectively. In vitro analysis indicated that an Fc fusion (vCD200-Fc) is expressed as a glycoprotein with a core molecular mass of 53 kDa. Utilizing monoclonal antibodies raised against vCD200-Fc, vCD200 expression was detected on the surfaces of and within supernatants from infected fibroblasts. Furthermore, in vitro assays demonstrated that vCD200-Fc treatment of monocyte-derived macrophages reduces tumor necrosis factor transcript and protein levels, implying that RRV encodes a functional vCD200.

* Corresponding author. Mailing address: Vaccine and Gene Therapy Institute, Oregon Health & Science University, West Campus, 505 N.W. 185th Avenue, Beaverton, OR 97006. Phone: (503) 690-5285. Fax: (503) 418-2719. E-mail: wongs{at}ohsu.edu.

Journal of Virology, March 2006, p. 3098-3103, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.3098-3103.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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