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Journal of Virology, March 2006, p. 2429-2436, Vol. 80, No. 5
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.5.2429-2436.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

MicroRNA-Guided Processing Impairs Plum Pox Virus Replication, but the Virus Readily Evolves To Escape This Silencing Mechanism

Carmen Simón-Mateo and Juan Antonio García*

Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain

Received 13 September 2005/ Accepted 6 December 2005

Since the discovery of microRNA (miRNA)-guided processing, a new type of RNA silencing, the possibility that such a mechanism could play a role in virus defense has been proposed. In this work, we have analyzed whether Plum pox virus (PPV) chimeras bearing miRNA target sequences (miR171, miR167, and miR159), which have been reported to be functional in Arabidopsis, were affected by miRNA function in three different host plants. Some of these PPV chimeras had clearly impaired infectivity compared with those carrying nonfunctional miRNA target sequences. The behaviors of PPV chimeras were similar but not identical in all the plants tested, and the deleterious effect on virus infectivity depended on the miRNA sequence cloned and on the site of insertion in the viral genome. The effect of the miRNA target sequence was drastically alleviated in transgenic plants expressing the silencing suppressor P1/HCPro. Furthermore, we show that virus chimeras readily escape RNA silencing interference through mutations within the miRNA target sequence, which mainly affected nucleotides matching the 5'-terminal region of the miRNA.

* Corresponding author. Mailing address: Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain. Phone: 34-915854535. Fax: 34-915854506. E-mail: jagarcia{at}cnb.uam.es.

Journal of Virology, March 2006, p. 2429-2436, Vol. 80, No. 5
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.5.2429-2436.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Duan, C.-G., Wang, C.-H., Fang, R.-X., Guo, H.-S. (2008). Artificial MicroRNAs Highly Accessible to Targets Confer Efficient Virus Resistance in Plants. J. Virol. 82: 11084-11095 [Abstract] [Full Text]  
  • Qu, J., Ye, J., Fang, R. (2007). Artificial MicroRNA-Mediated Virus Resistance in Plants. J. Virol. 81: 6690-6699 [Abstract] [Full Text]  
  • Valli, A., Martin-Hernandez, A. M., Lopez-Moya, J. J., Garcia, J. A. (2006). RNA Silencing Suppression by a Second Copy of the P1 Serine Protease of Cucumber Vein Yellowing Ipomovirus, a Member of the Family Potyviridae That Lacks the Cysteine Protease HCPro.. J. Virol. 80: 10055-10063 [Abstract] [Full Text]  
  • Vaucheret, H. (2006). Post-transcriptional small RNA pathways in plants: mechanisms and regulations.. Genes Dev. 20: 759-771 [Abstract] [Full Text]  


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