This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujita, R.
Right arrow Articles by Bando, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujita, R.
Right arrow Articles by Bando, H.

 Previous Article  |  Next Article 

Journal of Virology, March 2006, p. 2390-2395, Vol. 80, No. 5
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.5.2390-2395.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression of Autographa californica Multiple Nucleopolyhedrovirus Genes in Mammalian Cells and Upregulation of the Host ß-Actin Gene

Ryosuke Fujita,1 Takahiro Matsuyama,1,2 Junya Yamagishi,1,3 Ken Sahara,1 Shinichiro Asano,1 and Hisanori Bando1*

Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 001-8589, Japan,1 Research & Development Center, Santen Pharmaceutical Co., Ltd., Takayama-cho Ikoma-shi, Nara 630-0101, Japan,2 Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, New York 14853-18013

Received 29 September 2005/ Accepted 6 December 2005

The gene expression of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) was examined in two types of mammalian cells, human HeLa14 and hamster BHK cells. DNA microarray analysis followed by reverse transcription-PCR identified at least 12 viral genes transcribed in both HeLa14 cells and BHK cells inoculated with AcMNPV. 5' rapid amplification of cDNA ends was carried out to examine the transcriptional fidelity of these genes in HeLa14 cells. The transcription of ie-1, ie-0 and gp64 was initiated at a baculovirus early gene motif, CAGT, accompanied by a TATA motif. In addition, the same splicing observed for ie-0 mRNA in Sf9 cells occurred in HeLa14 cells. While the transcription initiation sites for pe38 and p6.9 were not located in the CAGT motif, most of them were in a typical eukaryotic RNA polymerase II promoter structure (a conventional TATA motif and/or an initiator). Interestingly, the expression of ß-actin was upregulated in the mammalian cells inoculated with AcMNPV. Subsequent experiments using UV-inactivated virus confirmed the upregulation, suggesting that de novo synthesis of viral products is not required for the event. These results indicated that the AcMNPV genome acts as a template for transcription in mammalian cells through the usual infection pathway, though there is no evidence for the functional expression of viral genes at present.

* Corresponding author. Mailing address: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 001-8589, Japan. Phone: 81-11 706-2487. Fax: 81-11 716-0879. E-mail: hban{at}abs.agr.hokudai.ac.jp.

Journal of Virology, March 2006, p. 2390-2395, Vol. 80, No. 5
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.5.2390-2395.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Abe, T., Kaname, Y., Wen, X., Tani, H., Moriishi, K., Uematsu, S., Takeuchi, O., Ishii, K. J., Kawai, T., Akira, S., Matsuura, Y. (2009). Baculovirus Induces Type I Interferon Production through Toll-Like Receptor-Dependent and -Independent Pathways in a Cell-Type-Specific Manner. J. Virol. 83: 7629-7640 [Abstract] [Full Text]  
  • Liu, C. Y.-Y., Wang, C.-H., Hsiao, W.-K., Lo, H.-R., Wu, C. P., Chao, Y. C. (2009). RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells. J. Virol. 83: 3604-3616 [Abstract] [Full Text]  
  • Liu, C. Y. Y., Wang, C. H., Wang, J. C., Chao, Y. C. (2007). Stimulation of baculovirus transcriptome expression in mammalian cells by baculoviral transcriptional activators. J. Gen. Virol. 88: 2176-2184 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»