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Journal of Virology, March 2006, p. 2127-2140, Vol. 80, No. 5
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.5.2127-2140.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Vaccinia Virus Proteome: Identification of Proteins in Vaccinia Virus Intracellular Mature Virion Particles

Che-Sheng Chung,1,{dagger} Chein-Hung Chen,2,{dagger} Ming-Yi Ho,2 Cheng-Yen Huang,1 Chung-Lin Liao,2* and Wen Chang1*

Institute of Molecular Biology,1 The Genomics Research Center, Academia Sinica, Taipei, Taiwan, Republic of China2

Received 25 October 2005/ Accepted 5 December 2005

Vaccinia virus is a large enveloped poxvirus with more than 200 genes in its genome. Although many poxvirus genomes have been sequenced, knowledge of the host and viral protein components of the virions remains incomplete. In this study, we used gel-free liquid chromatography and tandem mass spectroscopy to identify the viral and host proteins in purified vaccinia intracellular mature virions (IMV). Analysis of the proteins in the IMV showed that it contains 75 viral proteins, including structural proteins, enzymes, transcription factors, and predicted viral proteins not known to be expressed or present in the IMV. We also determined the relative abundances of the individual protein components in the IMV. Finally, 23 IMV-associated host proteins were also identified. This study provides the first comprehensive structural analysis of the infectious vaccinia virus IMV.

* Corresponding author. Mailing address for W. Chang: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China. Phone: 886-2-2789-9230. Fax: 886-2-2782-6085. E-mail: mbwen{at}ccvax.sinica.edu.tw. Mailing address for C.-L. Liao: The Genomics Research Center, Academia Sinica, Taipei, Taiwan, Republic of China. Phone: 886-2-2652-3926. Fax: 886-2-2782-9143. E-mail: clliao{at}gate.sinica.edu.tw.

{dagger} Che-Sheng Chung and Chein-Hung Chen contributed equally to this work.

Journal of Virology, March 2006, p. 2127-2140, Vol. 80, No. 5
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.5.2127-2140.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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