This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parez, N.
Right arrow Articles by Garbarg-Chenon, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parez, N.
Right arrow Articles by Garbarg-Chenon, A.

 Previous Article  |  Next Article 

Journal of Virology, February 2006, p. 1752-1761, Vol. 80, No. 4
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.4.1752-1761.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rectal Immunization with Rotavirus Virus-Like Particles Induces Systemic and Mucosal Humoral Immune Responses and Protects Mice against Rotavirus Infection{dagger}

Nathalie Parez,1,2,3* Cynthia Fourgeux,4 Ali Mohamed,4 Catherine Dubuquoy,4 Mathieu Pillot,4 Axelle Dehee,3 Annie Charpilienne,2 Didier Poncet,2 Isabelle Schwartz-Cornil,4,{ddagger} and Antoine Garbarg-Chenon3,{ddagger}

Service des Urgences Médicales Pédiatriques, Hôpital Armand Trousseau AP-HP, Paris,1 Unité de Virologie Moléculaire et Structurale, UMR CNRS 2472—INRA 1157, Gif sur Yvette,2 Laboratoire de Virologie, Université Pierre et Marie Curie-Paris6, EA 3500, AP-HP, Hôpital St. Antoine, Paris,3 Unité de Virologie et d'Immunologie Moléculaires, INRA, Jouy en Josas, France4

Received 8 July 2005/ Accepted 2 December 2005

To evaluate whether the rectal route of immunization may be used to provide appropriate protection against enteric pathogens such as rotaviruses (RV), we studied the antibody response and the protection induced by rectal immunization of mice with RV virus-like particles (VLP). For this purpose, 6-week-old BALBc mice were rectally immunized twice with RV 8-2/6/7-VLP derived from the bovine RV RF81 strain either alone or combined with various adjuvants including four toxins [cholera toxin (CT) and three attenuated Escherichia coli-derived heat-labile toxins (LTs), LT(R192G), LT(R72), and LT(K63)] and two Toll-like receptor-targeting adjuvants (CpG and resiquimod). Six weeks after the second immunization, mice were challenged with murine RV strain ECw. RV VLP administered alone were not immunogenic and did not protect mice against RV challenge. By contrast, RV VLP combined with any of the toxin adjuvants were immunogenic (mice developed significant titers of anti-RV immunoglobulin A [IgA] in both serum and feces and of anti-RV IgG in serum) and either efficiently induced complete protection of the mice (no detectable fecal virus shedding) or, for LT(K63), reduced the amount of fecal virus shedding after RV challenge. When combined with RV VLP, CpG and resiquimod failed to achieve protection, although CpG efficiently induced an antibody response to RV. These results support the consideration of the rectal route for the development of new immunization strategies against RV infection. Rectal delivery of a VLP-based vaccine might allow the use of adjuvants less toxic than, but as efficient as, CT.

* Corresponding author. Mailing address: Service des Urgences Médicales Pédiatriques, Hôpital Armand Trousseau, 26 ave. du Dr Arnold Netter, 75571 Paris cedex 12, France. Phone: 33 1 4473 6487. Fax: 33 1 4473 6985. E-mail: nathalie.parez{at}trs.aphp.fr.

{dagger} Dedicated to the memory of Jean Cohen, our close friend and mentor.

{ddagger} I.S.-C. and A.G.-C. share senior authorship.

Journal of Virology, February 2006, p. 1752-1761, Vol. 80, No. 4
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.4.1752-1761.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jiang, J. Q., He, X.-S., Feng, N., Greenberg, H. B. (2008). Qualitative and Quantitative Characteristics of Rotavirus-Specific CD8 T Cells Vary Depending on the Route of Infection. J. Virol. 82: 6812-6819 [Abstract] [Full Text]  
  • Lacasse, P., Denis, J., Lapointe, R., Leclerc, D., Lamarre, A. (2008). Novel Plant Virus-Based Vaccine Induces Protective Cytotoxic T-Lymphocyte-Mediated Antiviral Immunity through Dendritic Cell Maturation. J. Virol. 82: 785-794 [Abstract] [Full Text]  
  • Di Martino, C., Basset, C., Ogier, A., Charpilienne, A., Poncet, D., Kohli, E. (2007). Distribution and phenotype of rotavirus-specific B cells induced during the antigen-driven primary response to 2/6 virus-like particles administered by the intrarectal and the intranasal routes. J. Leukoc. Biol. 82: 821-828 [Abstract] [Full Text]  
  • Corthesy, B., Benureau, Y., Perrier, C., Fourgeux, C., Parez, N., Greenberg, H., Schwartz-Cornil, I. (2006). Rotavirus Anti-VP6 Secretory Immunoglobulin A Contributes to Protection via Intracellular Neutralization but Not via Immune Exclusion. J. Virol. 80: 10692-10699 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»