This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Avadhanula, V.
Right arrow Articles by Adderson, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Avadhanula, V.
Right arrow Articles by Adderson, E. E.

 Previous Article  |  Next Article 

Journal of Virology, February 2006, p. 1629-1636, Vol. 80, No. 4
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.4.1629-1636.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Respiratory Viruses Augment the Adhesion of Bacterial Pathogens to Respiratory Epithelium in a Viral Species- and Cell Type-Dependent Manner

Vasanthi Avadhanula,1,2 Carina A. Rodriguez,1,{dagger} John P. DeVincenzo,3,4 Yan Wang,1 Richard J. Webby,1 Glen C. Ulett,1,{ddagger} and Elisabeth E. Adderson1,2,3*

Department of Infectious Diseases, St. Jude Children's Research Hospital,1 Departments of Molecular Sciences,2 Pediatrics, University of Tennessee, Memphis,3 Children's Foundation Research Center, LeBonheur Children's Medical Center, Memphis, Tennessee4

Received 7 September 2005/ Accepted 1 December 2005

Secondary bacterial infections often complicate respiratory viral infections, but the mechanisms whereby viruses predispose to bacterial disease are not completely understood. We determined the effects of infection with respiratory syncytial virus (RSV), human parainfluenza virus 3 (HPIV-3), and influenza virus on the abilities of nontypeable Haemophilus influenzae and Streptococcus pneumoniae to adhere to respiratory epithelial cells and how these viruses alter the expression of known receptors for these bacteria. All viruses enhanced bacterial adhesion to primary and immortalized cell lines. RSV and HPIV-3 infection increased the expression of several known receptors for pathogenic bacteria by primary bronchial epithelial cells and A549 cells but not by primary small airway epithelial cells. Influenza virus infection did not alter receptor expression. Paramyxoviruses augmented bacterial adherence to primary bronchial epithelial cells and immortalized cell lines by up-regulating eukaryotic cell receptors for these pathogens, whereas this mechanism was less significant in primary small airway epithelial cells and in influenza virus infections. Respiratory viruses promote bacterial adhesion to respiratory epithelial cells, a process that may increase bacterial colonization and contribute to disease. These studies highlight the distinct responses of different cell types to viral infection and the need to consider this variation when interpreting studies of the interactions between respiratory cells and viral pathogens.

* Corresponding author. Mailing address: Room E8054, Mailstop 320, 332 N. Lauderdale St., Memphis, TN 38105. Phone: (901) 495-3459. Fax: (901) 495-3009. E-mail: elisabeth.adderson{at}stjude.org.

{dagger} Present address: Department of Pediatrics, University of South Florida, Tampa, Fla.

{ddagger} Present address: School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia.

Journal of Virology, February 2006, p. 1629-1636, Vol. 80, No. 4
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.4.1629-1636.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kukavica-Ibrulj, I., Hamelin, M.-E., Prince, G. A., Gagnon, C., Bergeron, Y., Bergeron, M. G., Boivin, G. (2009). Infection with Human Metapneumovirus Predisposes Mice to Severe Pneumococcal Pneumonia. J. Virol. 83: 1341-1349 [Abstract] [Full Text]  
  • Sajjan, U., Wang, Q., Zhao, Y., Gruenert, D. C., Hershenson, M. B. (2008). Rhinovirus Disrupts the Barrier Function of Polarized Airway Epithelial Cells. Am. J. Respir. Crit. Care Med. 178: 1271-1281 [Abstract] [Full Text]  
  • Sethi, S., Murphy, T. F. (2008). Infection in the Pathogenesis and Course of Chronic Obstructive Pulmonary Disease. NEJM 359: 2355-2365 [Full Text]  
  • Miyairi, I., DeVincenzo, J. P. (2008). Human Genetic Factors and Respiratory Syncytial Virus Disease Severity. Clin. Microbiol. Rev. 21: 686-703 [Abstract] [Full Text]  
  • Cardozo, D. M., Nascimento-Carvalho, C. M., Andrade, A.-L. S. S., Silvany-Neto, A. M., Daltro, C. H. C., Brandao, M.-A. S., Brandao, A. P., Brandileone, M.-C. C. (2008). Prevalence and risk factors for nasopharyngeal carriage of Streptococcus pneumoniae among adolescents. J Med Microbiol 57: 185-189 [Abstract] [Full Text]  
  • Bookwalter, J. E., Jurcisek, J. A., Gray-Owen, S. D., Fernandez, S., McGillivary, G., Bakaletz, L. O. (2008). A Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Homologue Plays a Pivotal Role in Nontypeable Haemophilus influenzae Colonization of the Chinchilla Nasopharynx via the Outer Membrane Protein P5-Homologous Adhesin. Infect. Immun. 76: 48-55 [Abstract] [Full Text]  
  • Martinez, F. J. (2007). Pathogen-directed Therapy in Acute Exacerbations of Chronic Obstructive Pulmonary Disease. Proc Am Thorac Soc 4: 647-658 [Abstract] [Full Text]  
  • Avadhanula, V., Wang, Y., Portner, A., Adderson, E. (2007). Nontypeable Haemophilus influenzae and Streptococcus pneumoniae bind respiratory syncytial virus glycoprotein. J Med Microbiol 56: 1133-1137 [Abstract] [Full Text]  
  • Teughels, W., Sliepen, I., Quirynen, M., Haake, S. K., Van Eldere, J., Fives-Taylor, P., Van Ranst, M. (2007). Human Cytomegalovirus Enhances A. actinomycetemcomitans Adherence to Cells. JDR 86: 175-180 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»