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Journal of Virology, February 2006, p. 1604-1609, Vol. 80, No. 3
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.3.1604-1609.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Two Internal Cleavage and Polyadenylation Sites of Parvovirus B19 RNA

Yuko Yoto, Jianming Qiu, and David J. Pintel^*

Department of Molecular Microbiology and Immunology, Life Sciences Center, University of Missouri-Columbia, School of Medicine, Columbia, Missouri

Received 12 September 2005/ Accepted 7 November 2005

Polyadenylation of B19 pre-mRNAs at the major internal site, (pA)p1, is programmed by the nonconsensus core cleavage and polyadenylation specificity factor-binding hexanucleotide AUUAAA. Efficient use of this element requires both downstream and upstream cis-acting elements and is further influenced by an adjacent AAUAAC motif. The primary hexanucleotide element must be nonconsensus to allow efficient readthrough of P6-generated pre-mRNAs into the capsid-coding region. An additional cleavage and polyadenylation site, (pA)p2, 296 nucleotides downstream of (pA)p1 was shown to be used following both B19 infection and transfection of a genomic clone. RNAs polyadenylated at (pA)p2 comprise approximately 10% of B19 RNAs that are polyadenylated internally.

Present address: Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan.

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