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Journal of Virology, December 2006, p. 12387-12392, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01232-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Efficient Reactivation of Latent Herpes Simplex Virus from Mouse Central Nervous System Tissues{triangledown}

Shih-Heng Chen,1 Hui-Wen Yao,1 Wen-Yen Huang,1 Kuei-Sen Hsu,1,2 Huan-Yao Lei,1,3 Ai-Li Shiau,1,3 and Shun-Hua Chen1,3*

Institute of Basic Medical Sciences,1 Department of Pharmacology,2 Department of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China3

Received 12 June 2006/ Accepted 14 September 2006

For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before explant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan 701, Republic of China. Phone: 886-6-2353535, ext. 5633. Fax: 886-6-2082705. E-mail: shunhua{at}mail.ncku.edu.tw.

{triangledown} Published ahead of print on 27 September 2006.

Journal of Virology, December 2006, p. 12387-12392, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01232-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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