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Journal of Virology, December 2006, p. 12357-12366, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01207-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of a Novel 5' Subgenomic RNA3a Derived from RNA3 of Brome Mosaic Bromovirus{triangledown}

Rafal Wierzchoslawski ,1,{dagger},{ddagger} Anna Urbanowicz,2,{ddagger} Aleksandra Dzianott,1 Marek Figlerowicz,2 and Jozef J. Bujarski1,2*

Plant Molecular Biology Center and the Department of Biological Sciences, Northern Illinois University, De Kalb, Illinois 60115,1 Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland2

Received 9 June 2006/ Accepted 19 September 2006

The synthesis of 3' subgenomic RNA4 (sgRNA4) by initiation from an internal sg promoter in the RNA3 segment was first described for Brome mosaic bromovirus (BMV), a model tripartite positive-sense RNA virus (W. A. Miller, T. W. Dreher, and T. C. Hall, Nature 313:68-70, 1985). In this work, we describe a novel 5' sgRNA of BMV (sgRNA3a) that we propose arises by premature internal termination and that encapsidates in BMV virions. Cloning and sequencing revealed that, unlike any other BMV RNA segment, sgRNA3a carries a 3' oligo(A) tail, in which respect it resembles cellular mRNAs. Indeed, both the accumulation of sgRNA3a in polysomes and the synthesis of movement protein 3a in in vitro systems suggest active functions of sgRNA3a during protein synthesis. Moreover, when copied in the BMV replicase in vitro reaction, the minus-strand RNA3 template generated the sgRNA3a product, likely by premature termination at the minus-strand oligo(U) tract. Deletion of the oligo(A) tract in BMV RNA3 inhibited synthesis of sgRNA3a during infection. We propose a model in which the synthesis of RNA3 is terminated prematurely near the sg promoter. The discovery of 5' sgRNA3a sheds new light on strategies viruses can use to separate replication from the translation functions of their genomic RNAs.

* Corresponding author. Mailing address: Plant Molecular Biology Center and the Department of Biological Sciences, Montgomery Hall, Northern Illinois University, De Kalb, IL 60115. Phone: (815) 753-0601. Fax: (815) 753-7855. E-mail: jbujarski{at}niu.edu.

{triangledown} Published ahead of print on 27 September 2006.

{dagger} Present address: National Institutes of Health, Fort Frederick, MD.

{ddagger} These two authors equally contributed to this work.

Journal of Virology, December 2006, p. 12357-12366, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01207-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yi, G., Letteney, E., Kim, C.-H., Kao, C. C. (2009). Brome mosaic virus capsid protein regulates accumulation of viral replication proteins by binding to the replicase assembly RNA element. RNA 15: 615-626 [Abstract] [Full Text]  
  • Sztuba-Solinska, J., Bujarski, J. J. (2008). Insights into the Single-Cell Reproduction Cycle of Members of the Family Bromoviridae: Lessons from the Use of Protoplast Systems. J. Virol. 82: 10330-10340 [Full Text]  


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