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Journal of Virology, November 2006, p. 11404-11408, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01102-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Antibody Binding Is a Dominant Determinant of the Efficiency of Human Immunodeficiency Virus Type 1 Neutralization{triangledown}

Xinzhen Yang,1,2* Inna Lipchina,1 Simon Cocklin,3 Irwin Chaiken,3 and Joseph Sodroski1,2,4

Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute,1 Department of Pathology, Division of AIDS, Harvard Medical School,2 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115,4 Department of Biochemistry & Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 191023

Received 30 May 2006/ Accepted 30 August 2006

Primary and laboratory-adapted variants of human immunodeficiency virus type 1 (HIV-1) exhibit a wide range of sensitivities to neutralization by antibodies directed against the viral envelope glycoproteins. An antibody directed against an artificial FLAG epitope inserted into the envelope glycoproteins of three HIV-1 isolates with vastly different neutralization sensitivities inhibited all three viruses equivalently. Thus, naturally occurring HIV-1 isolates that are neutralization resistant are not necessarily more impervious to the inhibitory consequences of bound antibody. Moreover, the binding affinity of the anti-FLAG antibody correlated with neutralizing potency, underscoring the dominant impact on neutralization of antibody binding to the envelope glycoproteins.

* Corresponding author. Present address: Beth Israel Deaconess Medical Center, 330 Brookline Avenue, R.E. 213A, Boston, MA 02215. Phone: (617) 667-2052. Fax: (617) 667-8210. E-mail: xyang1{at}bidmc.harvard.edu.

{triangledown} Published ahead of print on 6 September 2006.

Journal of Virology, November 2006, p. 11404-11408, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01102-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Magnus, C., Rusert, P., Bonhoeffer, S., Trkola, A., Regoes, R. R. (2009). Estimating the Stoichiometry of Human Immunodeficiency Virus Entry. J. Virol. 83: 1523-1531 [Abstract] [Full Text]  
  • Sheppard, N. C., Davies, S. L., Jeffs, S. A., Vieira, S. M., Sattentau, Q. J. (2007). Production and Characterization of High-Affinity Human Monoclonal Antibodies to Human Immunodeficiency Virus Type 1 Envelope Glycoproteins in a Mouse Model Expressing Human Immunoglobulins. CVI 14: 157-167 [Abstract] [Full Text]  


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