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Journal of Virology, November 2006, p. 11322-11330, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.00924-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

DNA Helicase Activity Is Associated with the Replication Initiator Protein Rep of Tomato Yellow Leaf Curl Geminivirus{triangledown}

Danielle Clérot and Françoise Bernardi*

Laboratoire de Biologie Moléculaire des Virus, Institut des Sciences du Végétal, CNRS, 91198 Gif-sur-Yvette Cedex, France

Received 5 May 2006/ Accepted 24 August 2006

The Rep protein of tomato yellow leaf curl Sardinia virus (TYLCSV), a single-stranded DNA virus of plants, is the replication initiator essential for virus replication. TYLCSV Rep has been classified among ATPases associated with various cellular activities (AAA+ ATPases), in superfamily 3 of small DNA and RNA virus replication initiators whose paradigmatic member is simian virus 40 large T antigen. Members of this family are DNA- or RNA-dependent ATPases with helicase activity necessary for viral replication. Another distinctive feature of AAA+ ATPases is their quaternary structure, often composed of hexameric rings. TYLCSV Rep has ATPase activity, but the helicase activity, which is instrumental in further characterization of the mechanism of rolling-circle replication used by geminiviruses, has been a longstanding question. We present results showing that TYLCSV Rep lacking the 121 N-terminal amino acids has helicase activity comparable to that of the other helicases: requirements for a 3' overhang and 3'-to-5' polarity of unwinding, with some distinct features and with a minimal AAA+ ATPase domain. We also show that the helicase activity is dependent on the oligomeric state of the protein.

* Corresponding author. Mailing address: Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France. Phone: 33 1 69 82 38 34. Fax: 33 (0)1 69 82 36 95. E-mail: francoise.bernardi{at}isv.cnrs-gif.fr.

{triangledown} Published ahead of print on 30 August 2006.

Journal of Virology, November 2006, p. 11322-11330, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.00924-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kittelmann, K., Rau, P., Gronenborn, B., Jeske, H. (2009). Plant Geminivirus Rep Protein Induces Rereplication in Fission Yeast. J. Virol. 83: 6769-6778 [Abstract] [Full Text]  
  • Arguello-Astorga, G., Ascencio-Ibanez, J. T., Dallas, M. B., Orozco, B. M., Hanley-Bowdoin, L. (2007). High-Frequency Reversion of Geminivirus Replication Protein Mutants during Infection. J. Virol. 81: 11005-11015 [Abstract] [Full Text]  
  • Choudhury, N. R., Malik, P. S., Singh, D. K., Islam, M. N., Kaliappan, K., Mukherjee, S. K. (2006). The oligomeric Rep protein of Mungbean yellow mosaic India virus (MYMIV) is a likely replicative helicase. Nucleic Acids Res 34: 6362-6377 [Abstract] [Full Text]  


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