JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JVI.01441-06v1
80/22/11235    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Antinone, S. E.
Right arrow Articles by Smith, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Antinone, S. E.
Right arrow Articles by Smith, G. A.

 Previous Article  |  Next Article 

Journal of Virology, November 2006, p. 11235-11240, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01441-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Two Modes of Herpesvirus Trafficking in Neurons: Membrane Acquisition Directs Motion{triangledown}

Sarah E. Antinone and Gregory A. Smith*

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois

Received 7 July 2006/ Accepted 1 September 2006

Alphaherpesvirus infection of the mammalian nervous system is dependent upon the long-distance intracellular transport of viral particles in axons. How viral particles are effectively trafficked in axons to either sensory ganglia following initial infection or back out to peripheral sites of innervation following reactivation remains unknown. The mechanism of axonal transport has, in part, been obscured by contradictory findings regarding whether capsids are transported in axons in the absence of membrane components or as enveloped virions. By imaging actively translocated viral structural components in living peripheral neurons, we demonstrate that herpesviruses use two distinct pathways to move in axons. Following entry into cells, exposure of the capsid to the cytosol resulted in efficient retrograde transport to the neuronal cell body. In contrast, progeny virus particles moved in the anterograde direction following acquisition of virion envelope proteins and membrane lipids. Retrograde transport was effectively shut down in this membrane-bound state, allowing for efficient delivery of progeny viral particles to the distal axon. Notably, progeny viral particles that lacked a membrane were misdirected back to the cell body. These findings show that cytosolic capsids are trafficked to the neuronal cell body and that viral egress in axons occurs after capsids are enshrouded in a membrane envelope.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Ward, Rm. 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Phone: (312) 503-1339. Fax: (312) 503-1339. E-mail: g-smith3{at}northwestern.edu.

{triangledown} Published ahead of print on 13 September 2006.

Journal of Virology, November 2006, p. 11235-11240, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01441-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2006 by the American Society for Microbiology. All rights reserved.