This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, F.
Right arrow Articles by Dixon, L. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, F.
Right arrow Articles by Dixon, L. K.

 Previous Article  |  Next Article 

Journal of Virology, November 2006, p. 10514-10521, Vol. 80, No. 21
0022-538X/06/$08.00+0     doi:10.1128/JVI.00485-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Macrophage Transcriptional Responses following In Vitro Infection with a Highly Virulent African Swine Fever Virus Isolate

Fuquan Zhang,1,# Paul Hopwood,2,# Charles C. Abrams,1 Alison Downing,3 Frazer Murray,3 Richard Talbot,3 Alan Archibald,3 Stewart Lowden,2 and Linda K. Dixon1*

Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 ONF, United Kingdom,1 ARK-Genomics Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom,2 Royal Dick Veterinary College, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, United Kingdom3

Received 8 March 2006/ Accepted 31 July 2006

We used a porcine microarray containing 2,880 cDNAs to investigate the response of macrophages to infection by a virulent African swine fever virus (ASFV) isolate, Malawi LIL20/1. One hundred twenty-five targets were found to be significantly altered at either or both 4 h and 16 h postinfection compared with targets after mock infection. These targets were assigned into three groups according to their temporal expression profiles. Eighty-six targets showed increased expression levels at 4 h postinfection but returned to expression levels similar to those in mock-infected cells at 16 h postinfection. These encoded several proinflammatory cytokines and chemokines, surface proteins, and proteins involved in cell signaling and trafficking pathways. Thirty-four targets showed increased expression levels at 16 h postinfection compared to levels at 4 h postinfection and in mock-infected cells. One host gene showed increased expression levels at both 4 and 16 h postinfection compared to levels in mock-infected cells. The microarray results were validated for 12 selected genes by quantitative real-time PCR. Levels of protein expression and secretion were measured for two proinflammatory cytokines, interleukin 1ß and tumor necrosis factor alpha, during a time course of infection with either the virulent Malawi LIL20/1 isolate or the OUR T88/3 nonpathogenic isolate. The results revealed differences between these two ASFV isolates in the amounts of these cytokines secreted from infected cells.

* Corresponding author. Mailing address: Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 ONF, United Kingdom. Phone: 44 1483 231062. Fax: 44 1483 232448. E-mail: linda.dixon{at}bbsrc.ac.uk.

# F. Zhang and P. Hopwood contributed equally to this work.

Journal of Virology, November 2006, p. 10514-10521, Vol. 80, No. 21
0022-538X/06/$08.00+0     doi:10.1128/JVI.00485-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»