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Journal of Virology, November 2006, p. 10457-10464, Vol. 80, No. 21
0022-538X/06/$08.00+0     doi:10.1128/JVI.00892-06

ORF3 Protein of Hepatitis E Virus Is Not Required for Replication, Virion Assembly, or Infection of Hepatoma Cells In Vitro{triangledown}

Suzanne U. Emerson,* Hanh Nguyen, Udana Torian, and Robert H. Purcell

Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892

Received 2 May 2006/ Accepted 15 August 2006

A subclone of Huh-7 cells that could be relatively efficiently transfected and infected with hepatitis E virus was identified. Following transfection, infectious virus was produced but remained predominantly cell associated. Intracellular virus, recovered by lysis of transfected cells, infected naïve cells. This in vitro-produced virus appeared to be antigenically identical to virus isolated from clinical samples. Lysates from cells transfected with mutant viral genomes unable to synthesize ORF3 protein contained infectious virions that were similar in number, thermostability, and sedimentation characteristics to those in lysates transfected with wild-type viral genomes. Therefore, in contrast to its requirement in vivo, ORF3 protein is not required for infection of Huh-7 cells or production of infectious virus in vitro.

* Corresponding author. Mailing address: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 50, Room 6537, 50 South Drive, MSC 8009, Bethesda, MD 20892. Phone: (301) 496-2787. Fax: (301) 402-0524. E-mail: semerson{at}niaid.nih.gov.

{triangledown} Published ahead of print on 23 August 2006.

Journal of Virology, November 2006, p. 10457-10464, Vol. 80, No. 21
0022-538X/06/$08.00+0     doi:10.1128/JVI.00892-06




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