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Journal of Virology, October 2006, p. 10262-10269, Vol. 80, No. 20
0022-538X/06/$08.00+0     doi:10.1128/JVI.00974-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Nuclear Import Defect of Human Immunodeficiency Virus Type 1 DNA Flap Mutants Is Not Dependent on the Viral Strain or Target Cell Type

Nathalie Arhel,{dagger} Sandie Munier,{dagger} Philippe Souque, Karine Mollier, and Pierre Charneau*

Virologie Moléculaire et Vectorologie, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris, France

Received 12 May 2006/ Accepted 24 July 2006

We have previously established, using human immunodeficiency virus type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear import. Although the impact of the DNA Flap on nuclear import has already found numerous independent confirmations in the context of lentivirus vectors, it has been claimed that it may be nonessential for infectious virus strains LAI, YU-2 (J. D. Dvorin et al., J. Virol. 76:12087-12096, 2002), HXB2, and NL4-3 (A. Limon et al., J. Virol. 76:12078-12086, 2002). We conducted a detailed analysis of virus infectivity using the provirus clones provided by the authors and analogous target cells. In contrast to published data, our results show that all cPPT mutant viruses exhibit reduced infectivity corresponding to a nuclear import defect irrespective of the viral genetic background or target cell.

* Corresponding author. Mailing address: Groupe de Virologie Moléculaire et Vectorologie, Département de Virologie, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris, France. Phone: 33 1 45 68 88 22. Fax: 33 1 40 61 34 65. E-mail: charneau{at}pasteur.fr.

{dagger} N.A. and S.M. contributed equally to this work.

Journal of Virology, October 2006, p. 10262-10269, Vol. 80, No. 20
0022-538X/06/$08.00+0     doi:10.1128/JVI.00974-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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