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Journal of Virology, January 2006, p. 794-801, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.794-801.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Involvement of Template-Activating Factor I/SET in Transcription of Adenovirus Early Genes as a Positive-Acting Factor

Hirohito Haruki,1 Mitsuru Okuwaki,1 Makoto Miyagishi,2 Kazunari Taira,2 and Kyosuke Nagata1*

Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305-8575, Japan,1 Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, Tokyo 113-8656, Japan2

Received 4 July 2005/ Accepted 21 October 2005

The adenovirus genome complexed with viral core protein VII (adenovirus DNA-protein VII complex) at least is the bona fide template for transcription of adenovirus early genes. It is believed that the highly basic protein VII, like cellular histones, is a negative regulator for genome functions. Analyses with in vitro replication and transcription systems using the adenovirus DNA-protein VII complex have revealed that remodeling of the complex is crucial for efficient DNA replication and transcription. We identified host acidic proteins, template-activating factor I (TAF-I), TAF-II, and TAF-III as stimulatory factors for replication from the adenovirus DNA-protein VII complex. Recently, it was reported that the adenovirus DNA interacts with TAF-I and pp32, another host acidic protein (Y. Xue, J. S. Johnson, D. A. Ornelles, J. Lieberman, and D. A. Engel, J. Virol. 79:2474-2483, 2005). We found that TAF-I interacts and colocalizes with protein VII in adenovirus-infected cells during the early phases of infection, but pp32 does not. Although pp32 had the potential ability to interact with protein VII, pp32 did not remodel the adenovirus DNA-protein VII complex in vitro. Small interfering RNA-mediated knockdown of TAF-I expression leads to the delay of the transcription timing of early genes. These results provide evidence that TAF-I plays an important role in the early stages of the adenovirus infection cycle.

* Corresponding author. Mailing address: Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan. Phone and fax: (81) 298-53-3233. E-mail: knagata{at}md.tsukuba.ac.jp.

Journal of Virology, January 2006, p. 794-801, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.794-801.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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